A kind of dna immunoadsorbent and preparation method thereof
An immunoadsorbent and pore adsorption technology, applied in the field of DNA immunoadsorbent and its preparation, can solve the problems of affecting the adsorption performance of the adsorbent, insufficient DNA encapsulation, decrease of DNA immobilization capacity, etc., so as to improve the use safety, The effect of less particle shedding and reduction of the amount of pre-flush
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0042] (1) Preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups:
[0043] In a 1000mL three-necked flask, add 500mL of aqueous solution containing 1.5wt% gelatin, add 7.5g of 4-vinylbenzaldehyde, 10.5g of ethyl styrene, 42g of divinylbenzene, 120g of cyclohexanol, and 0.6g of benzene peroxide For the mixed organic phase of formyl, under mechanical stirring, heat up to 75°C for polymerization reaction for 3 hours, heat up to 80°C for 7 hours, and then heat up to 85°C for 5 hours. Extract for 20 hours, wash with water until there is no smell of acetone, and filter with suction to obtain a polystyrene-based macroporous adsorption resin containing aldehyde groups, and screen out the resin with a particle size of 0.5-1.2 mm for future use.
[0044] The specific surface area, pore structure and other data were tested by nitrogen adsorption method, and it was known that the specific surface area of the obtained resin was 760m 2 / g; the pore volu...
Embodiment 2
[0050] (1) Preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups:
[0051] In a 1000mL three-necked flask, add 500mL of aqueous solution containing 1wt% polyvinyl alcohol, then add 15g of 3-vinylbenzaldehyde, 9g of ethyl styrene, 36g of divinylbenzene, 120g of toluene, and 0.6g of benzoyl peroxide. Mix the organic phase, heat up to 75°C for 3 hours under mechanical stirring, heat up to 80°C for 7 hours to solidify, then heat up to 85°C for 5 hours, after the reaction is over, evaporate the porogen toluene, wash with water until the washing solution is clear Finally, extract with acetone for 12 hours, wash with water until there is no smell of acetone, and filter with suction to obtain a polystyrene-based macroporous adsorption resin containing aldehyde groups. The resin with a particle size of 0.5-1.2 mm is selected for use.
[0052] The specific surface area, pore structure and other data were tested by nitrogen adsorption method, and it was...
Embodiment 3
[0058] (1) Preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups:
[0059] Add 500mL of aqueous solution containing 0.1wt% carboxymethylcellulose to a 1000mL three-necked flask, add 20g 4-vinylbenzaldehyde, 8g ethylstyrene, 32g divinylbenzene, 90g toluene, 60g liquid paraffin, 0.6g For the mixed organic phase of benzoyl peroxide, under mechanical stirring, heat up to 75°C for polymerization reaction for 5 hours, heat up to 80°C for 6 hours, then heat up to 85°C for 5 hours, after the reaction, steam the porogen After soaking in toluene, wash with water until the washing solution is clear, extract with acetone for 12 hours, then wash with water until there is no acetone smell, and filter with suction to obtain a polystyrene-based macroporous adsorption resin containing aldehyde groups, with a particle size of 0.5-1.2mm The resin is spare.
[0060] The specific surface area, pore structure and other data were tested by nitrogen adsorption meth...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
pore size | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com