Method for facilitating proliferation of adipose-derived stem cells
A technology of adipose stem cells and cells, which is applied in the field of promoting the proliferation of adipose stem cells, can solve the problems that cells cannot survive and proliferate well, and achieve the effect of high proliferation rate
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[0016] The technical solutions of the present invention are described below in combination with specific embodiments. The experimental materials that are not particularly emphasized in the following examples are conventional experimental materials without special requirements, and are all conventional materials that are easily obtained by those skilled in the art.
[0017] 1. Experimental materials
[0018] Saccharin A, Saccharin B, Saccharin F and Saccharin F were self-made according to the literature method, and the purity was greater than 98%.
[0019] DMEM medium and fetal bovine serum were purchased from Gibco. CCK-8 kit was purchased from colleagues in Japan.
[0020] 2. Experimental method
[0021] 1. Isolation and culture of adipose-derived stem cells
[0022] The subcutaneous adipose tissue of healthy volunteers was taken, washed three times with PBS buffer, and the adipose tissue membrane and blood vessels were removed with ophthalmic scissors and ophthalmic forc...
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