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Rotary injection device and method for single cells

An injection device and single-cell technology, applied in the field of cell injection, can solve problems such as the limitation of positioning accuracy and operation accuracy, and achieve the effects of reducing injection force, high test accuracy, and significant research value

Active Publication Date: 2021-06-18
香港城市大学深圳研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of cell microinjection automation, domestic and foreign research institutes have successively launched some cell microinjection platforms with their own advantages and disadvantages. However, due to the limitation of positioning accuracy and operation accuracy, the force of cell microinjection can be effectively reduced to reduce cell damage. is still a problem

Method used

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  • Rotary injection device and method for single cells
  • Rotary injection device and method for single cells
  • Rotary injection device and method for single cells

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Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0034] like figure 1 and image 3 As shown, the present invention provides a rotary injection device for single cells, wherein the rotary injection device for single cells includes a tilt angle adjustment slider 1 and a fixed-angle wedge-shaped connection block 2, the fixed-angle wedge-shaped connection The block 2 is fixed on the upper surface of the inclination angle adjustment slider 1, and a motor connection module 6 is movably provided above the fixed angle wedge-shaped connection block 2, and the motor connection module 6 is connected with a propelling straight line for injection. Motor 5, a rotary motor 8 for injection is provided above the motor connection module 6, and a rotary motor 8 for injection is movably provided with an injection needle 12, and the injection needle 12 can advance the linear motor 5 for injection and the injection needle 12. The cells 13 to be injected are rotated and injected under the action of the rotary motor 8 for injection.

[0035] Spec...

Embodiment approach 2

[0046] like figure 2 and image 3 As shown, the present invention provides a rotary injection method for single cells, wherein the rotary injection method for single cells adopts the above-mentioned rotary injection device for single cells. The single-cell rotary injection device has the same structure, working principle and beneficial effects as the first embodiment, and will not be repeated here. The single-cell rotary injection method includes the following steps: Step S1, moving the injection needle 12 to the injection site Use the rotation center of the rotating motor 8; Step S2, adjust the tilt angle adjustment slider 1, and set the injection angle; Step S3, move the motor connection module 6 to align the injection needle 12 with the cells to be injected 13; Step S4, while The injection needle 12 is used to rotate and inject the cells 13 to be injected by operating the injection propelling motor 5 and the injection rotating motor 8 .

[0047] Further, before the step ...

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Abstract

The invention relates to a rotary injection device and method for single cells. The device includes a tilt angle adjustment slider and a wedge-shaped connection block with a fixed angle. A motor connection module is movable above the wedge-shaped connection block with a fixed angle. The motor connection module is connected to There is a propulsion linear motor for injection, a rotary motor for injection is provided above the motor connection module, and an injection needle can be moved by the rotary motor for injection, and the injection needle rotates under the action of the linear propulsion motor for injection and the rotary motor for injection. Injecting cells; the method includes: Step S1, moving the injection needle to the rotation center of the rotating motor for injection; Step S2, adjusting the tilt angle adjustment slider to set the injection angle; Step S3, moving the motor connection module to make the injection needle and the Alignment of injected cells; step S4, simultaneously operate the propulsion motor for injection and the rotation motor for injection to perform rotary injection. The invention can position and rotate single cells for injection, reducing the force of cell injection.

Description

technical field [0001] The invention relates to the field of cell injection, in particular to a rotary injection device and method for single cells. Background technique [0002] In a microscopic environment, a small amount of exogenous substances are injected into the cytoplasm or nucleus of a cell through a specific equipment platform and method, which is called cell injection. This technology is an important operation and research method in bioengineering research. It plays a key role in the manipulation of embryos, cells, chromosomes, DNA, etc. in bioengineering. [0003] In general, the entire cell microinjection process is mainly composed of five steps: preparation of cells to be injected, search for cells, adsorption and fixation, needle insertion into the membrane, and injection and withdrawal of the needle. Among them, in the cell microinjection stage of the acupuncture membrane, because the cells are soft, fragile and easily broken, the injection force of the cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N15/89
CPCC12M35/00C12N15/89
Inventor 申亚京陆豪健
Owner 香港城市大学深圳研究院