Preservation and propagation method of melon powdery mildew bacteria

Abstract

The invention discloses a preservation and propagation method for cucurbites sphaerotheca fuliginea. The method comprises the following steps: 1) in an aseptic condition, purifying the cucurbites sphaerotheca fuliginea, until a new bacterial colony appears; 2) infecting an arabidopsis plant by the new bacterial colony, and cultivating; and 3) replacing with a new arabidopsis plant, cultivating, and repeatedly performing the step to realize the preservation and the propagation of the cucurbites sphaerotheca fuliginea. The method is capable of guaranteeing long-term supply of the cucurbites sphaerotheca fuliginea, and providing great convenience for a correlational research of cucurbites powdery mildew; the arabidopsis plant is easy to plant and cultivate, the seed cost and the planting andcultivating cost are low, and an occupied space is small, so the problems that a long-term preservation and propagation process is tedious, the planting and maintaining cost is high and the occupied space is large are solved; in addition, the method is simple and easy, economic, convenient, and wide in applicability.

Description

technical field [0001] The invention relates to the technical field of plant pathology, in particular to a method for preserving and propagating melon powdery mildew bacteria. Background technique [0002] Cucurbit powdery mildew is a widespread worldwide disease, which mainly infects the leaves of melon plants such as cucumber, bitter gourd, pumpkin, watermelon, muskmelon, wax gourd, zucchini and cattail melon. The disease can occur at any stage; under suitable conditions, melon powdery mildew develops rapidly and spreads to the entire plant within a few days or a week, causing the leaves to turn yellow, lose function, and gradually die, seriously affecting the yield and quality of melon crops. quality. [0003] Cucurbit powdery mildew bacteria are mainly divided into the genus Podosphaeraxanthii (formerly known as S. fuliginea) and the genus Golorinomycescichoracearum (formerly known as E. cichoracearum), which are obligate parasites and can only parasitize On living hos...

AI Technical Summary

Problems solved by technology

Low-temperature in vitro storage (in liquid nitrogen or in a -80°C refrigerator) requires the use of liquid nitrogen or an ultra-low temperature refrigerator, which consumes a lot of resources, and with the extension of storage time, the germination rate of powdery mildew spores is low, thus losing the pathogenicity; Although the moisturizing preservation of in vivo leaves can be effectively preserved, it requires light cultivation and frequent replacement of leaves, which is a huge workload and is not conducive to long-term preservation; while in vivo preservation is usually inoculated with powdery mildew bacteria into the body or in a light incubator at room temperature or Preservation and propagation on other melon susceptible lines

However, due to the high cost of seeds of melon crops and the difficulty of germination, soaking and germination treatments are usually required; in addition, a lot of time and energy are required for seedling cultivation, planting and maintenance of melon crops, and the plants are large and take up a lot of space. The cost of planting is high. Therefore, in actual operation, people still rely on the natural diseased melon plants in the field to provide powdery mildew bacteria. However, this method is greatly affected by the environment and climate, which seriously affects and restricts the resistance to melon powdery mildew. The development of related research work such as appraisal, powdery mildew resistance gene positional cloning, powdery mildew fungus biology and fungicide development

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