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Marine aspergillus derived oxa-anthraquinone compound, preparation method thereof and application in preparation of antineoplastic agent

A technology of marine Aspergillus and xanthraquinones, applied in the application field of marine Aspergillus-derived xanthraquinone compounds and their preparation, and preparation of anti-tumor agents, which can solve the problems of limited species and anti-tumor activity, and achieve strong inhibition Active, broad application prospects

Active Publication Date: 2018-11-06
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to provide a marine aspergillus-derived xanthraquinone compound and its preparation method to solve the problem of limited species and antitumor activity of marine fungal-derived xanthraquinone compounds in the prior art

Method used

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  • Marine aspergillus derived oxa-anthraquinone compound, preparation method thereof and application in preparation of antineoplastic agent
  • Marine aspergillus derived oxa-anthraquinone compound, preparation method thereof and application in preparation of antineoplastic agent
  • Marine aspergillus derived oxa-anthraquinone compound, preparation method thereof and application in preparation of antineoplastic agent

Examples

Experimental program
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Effect test

Embodiment 1

[0050] (1) Cultivation of marine fungus ZA-01 strain

[0051] The medium used for the culture of marine fungus ZA-01 contains 1.0wt% glucose, 0.1wt% yeast extract, 0.2wt% peptone, 1.0wt% agar, 3.0wt% coarse sea salt, and the rest is water. It is made into a test tube when used For slant, the marine fungus ZA-01 strain was cultured at 28°C for 3 days.

[0052] (2) Fermentation and cultivation of marine fungus ZA-01

[0053] The fermentation medium used for the fermentation culture of the marine fungus ZA-01 is that each 1000mL Erlenmeyer flask contains 60g of rice, NaNO 3 0.1g, KH 2 PO 4 0.05g, MgSO 4 ·7H 2 O 0.02g, NaCl 0.02g, FeSO 4 0.01g, 2g sucrose, 50mL water, and the strain was fermented and cultured at 28°C for 28 days to obtain a fermented product; a total of 80 1000mL Erlenmeyer flasks were used for fermentation.

[0054] (3) Separation and analysis of xanthraquinone compounds

[0055] Take the fermented product obtained in step (2), extract twice with ethyl ac...

Embodiment 2

[0066] (1) Cultivation of marine fungus ZA-01 strain

[0067] The medium used for the culture of the marine fungus ZA-01 contains 10wt% of glucose, 4.0wt% of yeast extract, 4.0wt% of peptone, 6.0wt% of agar, 10wt% of coarse sea salt, and the rest is water. Fungal strains were grown at 35°C for 5 days.

[0068] (2) Fermentation and cultivation of marine fungus ZA-01

[0069] The fermentation medium used for the fermentation culture of the marine fungus ZA-01 is that each 1000mL Erlenmeyer flask contains 150g of rice, NaNO 3 0.6g, KH 2 PO 4 0.2g, MgSO 4 7H2O 0.01g, NaCl 0.1g, FeSO 4 0.05g, 6g of sucrose, and 150mL of water, the strain was fermented and cultivated at 35°C for 45 days to obtain a fermented product.

[0070] (3) Separation and analysis of xanthraquinone compounds

[0071] Take the fermented product obtained in step (2), extract 4 times with ethyl acetate, combine the ethyl acetate extracts and concentrate under reduced pressure to obtain a crude extract, fir...

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Abstract

The invention provides a marine aspergillus derived oxa-anthraquinone compound, a preparation method thereof and application in preparation of an antineoplastic agent. The marine aspergillus is a (Aspergillus sp.) ZA-01 strain, the preservation date is January 25, 2018, and the preservation number is CGMCC No.15270, the preservation unit is the general microbiology center of China Committee for Culture Collection of Microorganisms, and the address of the preservation unit is No.3, yard No.1, Beichen west road, Chaoyang district, Beijing. The structural formula of the compound is selected fromformulas 1-6, and the compound is separated from a fermentation product by fermenting the (Aspergillus sp.) ZA-01 strain. It is verified that the compound has very strong inhibitory activity on tumorcells, can be used as an anti-tumor drug and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of marine natural medicinal chemistry, in particular to a marine aspergillus-derived xanthraquinone compound, a preparation method thereof, and an application in the preparation of an antitumor agent. Background technique [0002] The ocean accounts for about 71% of the total surface area of ​​the earth. It contains rich and diverse biological resources and is a valuable resource bank for human drug research and development. Since the 1940s, a variety of marine-derived drugs represented by cephalosporins, cytarabine and ET-743 have been successfully used clinically. Marine-derived fungal microbial resources have become one of the most important sources of marine drugs because of their rich metabolites and repeatable fermentation. Among them, the anti-tumor active compounds represented by marine fungal-derived xanthraquinones have become the most important sources of modern marine drugs. important topic in drug resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18A61K31/352A61P35/00C12R1/66
CPCA61P35/00A61K31/352C12P17/18C12R2001/66C12N1/145
Inventor 曹飞朱华结朱奡刘莉
Owner HEBEI UNIVERSITY
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