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Kit for diagnosis of early non-small cell lung cancer

A technology for early diagnosis of non-small cell lung cancer, applied in the medical and health field, can solve problems such as undiscovered protein expression levels, achieve good diagnostic results, convenient and quick preparation, and simple extraction

Active Publication Date: 2018-11-13
郭伟
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No reports on protein expression levels in exosomes from metastatic non-small cell lung cancer have been found so far

Method used

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  • Kit for diagnosis of early non-small cell lung cancer
  • Kit for diagnosis of early non-small cell lung cancer
  • Kit for diagnosis of early non-small cell lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example

[0040] Implementation case: Application of AHSG and ECM-1 in the diagnosis of early non-small cell lung cancer

[0041] 1. Experimental design

[0042] The experiment was designed to collect serum samples from newly diagnosed patients with non-small cell lung cancer and healthy volunteers in Shandong Cancer Hospital, isolate and enrich exosomes and obtain exosome proteins. The expressions of AHSG and ECM-1 were detected by ELISA and western blot, and the differences in their expressions among healthy volunteers, non-small cell lung cancer and early non-small cell lung cancer were statistically analyzed, and their diagnostic efficacy alone and in combination with CEA were analyzed.

[0043] 2. Patients and samples involved in the experiment

[0044] 2 ml of serum samples were collected from 125 newly diagnosed patients with non-small cell lung cancer in Shandong Provincial Cancer Hospital, including 80 male patients and 45 female patients, with an average age of 60 years. The...

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PUM

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Abstract

The invention provides a kit for diagnosis of early non-small cell lung cancer. A marker of the kit is protein of serum exosomes, and the exosome protein is exosome human fetuin A, namely AHSG or / andexosome extracellular matrix protein-1, namely ECM-1. The content of the marker in a sample is detected, and if the content of the marker in the sample is greater than the critical value range thereof, a tester can preliminarily judge that the sample has larger risk of non-small cell lung cancer. The marker in the kit is simple to extract, the preparation is convenient and rapid, the detection process is simple, and the kit has a good diagnostic effect on early non-small cell lung cancer.

Description

technical field [0001] The invention relates to the development of a non-small cell lung cancer diagnostic kit based on exosomal proteins AHSG and ECM-1, belonging to the field of medical and health care. Background technique [0002] Non-small cell lung cancer (non-small cell lung cancer, non-small cell lung cancer) accounts for 80% of all lung cancers, about 75% of patients have distant metastasis at the time of treatment, and the 5-year survival rate is only 2%. Therefore, there is an urgent need for a more sensitive diagnostic marker to provide an objective basis for the early diagnosis, early treatment and prognosis of non-small cell lung cancer with distant metastasis. [0003] Tumor cell-derived exosomes (Tomor-derived exosomes, TDEs) have been shown to promote cancer progression, and the intercellular interactions mediated by exosomes may play an important role in tumorigenesis and distant metastasis. The isolation of exosomes and their use in the clinic may be an a...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57423
Inventor 郭伟刘利胜宋兴国
Owner 郭伟
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