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A technology of shimeji mushrooms and strains, applied in the field of strain breeding and production, can solve the problems of high input cost, complicated process, inconvenience for large-scale promotion, etc., and achieve the effect of easy promotion and no energy consumption.
Inactive Publication Date: 2019-01-11
江苏品品鲜生物科技股份有限公司
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[0003] The object of the present invention is to provide a kind of method for the selection and breeding of the genus jitake strains, to solve the above-mentioned background technology, the breeding of the existing shimeji mushroom strains is mainly through physical and chemical method mutagenesis to cultivate the resistance to Trichoderma viride. Vigorous jiji mushroom strains, this method is complicated in process, high in input cost, and not convenient for large-scale promotion
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Embodiment 1
[0024] A kind of breeding method of jiji mushroom bacterial classification, concrete breeding method step is as follows:
[0025] Step 1: Add pagoda seed dregs, Chinese herbal medicine mixture, pagoda tree powder, potassium hydrogen phosphate, pig bile salt, glucose, lactose, and agar into the medium;
[0026] Step 2: Add clean water to the culture medium in Step 1, shake well, and pour it into a petri dish;
[0027] Step 3: draw a black line on the bottom cover of each petri dish to divide the area into two equal parts, and inoculate the mother species of jiji mushroom and antagonistic bacteria respectively at two points 3cm away from the center of the plate;
[0028] Step 4: Control the temperature of the inoculated Petri dish at 22°C and keep it closed in the dark;
[0029] Step 5: After culturing, calculate the bacteriostatic rate of the mushroom strains in each petri dish to antagonistic bacteria, and select the mushroom strains in the culture dish with a higher inhibiti...
Embodiment 2
[0038] A kind of breeding method of jiji mushroom bacterial classification, concrete breeding method step is as follows:
[0039] Step 1: Add pagoda seed dregs, Chinese herbal medicine mixture, pagoda tree powder, superphosphate, gypsum, glucose, lactose, and agar into the medium;
[0040] Step 2: Add clean water to the culture medium in Step 1, shake well, and pour it into a petri dish;
[0041] Step 3: draw a black line on the bottom cover of each petri dish to divide the area into two equal parts, and inoculate the mother species of jiji mushroom and antagonistic bacteria respectively at two points 3cm away from the center of the plate;
[0042] Step 4: Control the temperature of the inoculated Petri dish at 22°C and keep it closed in the dark;
[0043] Step 5: After culturing, calculate the bacteriostatic rate of the mushroom strains in each petri dish to antagonistic bacteria, and select the mushroom strains in the culture dish with a higher inhibition rate to be the sel...
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Abstract
The invention discloses a breeding method for Hypsizygus marmoreus strains. The specific breeding method comprises the following steps: 1) adding residue of sophora japonica, mixture of Chinese herbalmedicine, sophora japonica powder, potassium hydrogen phosphate, pig bile salt, glucose, lactose and agar into a culture medium; 2), adding clean water into the culture medium of the step 1, shakingevenly, and pouring into a petri dish; 3), drawing a black line on the bottom cover of each petri dish to divide the area into two equal part, and inoculating mother species and antagonistic bacteriaof Hypsizygus marmoreus at two opposite points 3cm away from the center of the plate respectively; 4), controlling the temperature of the inoculated Petri dish to 22 DEG C, and culturing the inoculated Petri dish in a light-shielding and airtight manner; and 5), after culturing, calculate that bacteriostasis rate of the H. maritima strains to antagonistic bacteria in each culture dish, and selecting the H. maritima strains in the culture dish with high bacteriostasis rate as the selected target strain. The breeding method for Hypsizygus marmoreus strains is simple and convenient, and is easy to popularize. And basically does not consume energy, no pollution to the environment, in line with the national energy-saving and environmental protection industrial policy.
Description
technical field [0001] The invention belongs to the technical field of strain breeding and production, and in particular relates to a method for breeding strains of shimeji mushroom. Background technique [0002] The growth rate of jiji mushroom is slow, the ability to inhibit miscellaneous bacteria is poor, and it is easily affected by the cultivation environment and cultivation conditions, which has certain disadvantages compared with other factory-produced edible fungi. Breeding of strains is one of the most critical technologies in the cultivation steps of shiji mushroom. Due to the limitation of technology and equipment conditions, the cultivation of shiji mushroom is only limited to a small area, which is not conducive to the comprehensive promotion and popularization of shiji mushroom industry. The selection and breeding of the existing mushroom strains mainly adopt physical and chemical methods to mutagenize and cultivate the mushroom strains with strong activity aga...
Claims
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