Method for isolating edible fungus strains

A technology for edible fungi and strains, applied in the fields of botanical equipment and methods, applications, gardening, etc., can solve the problems of easy movement of tissue blocks, increase the chance of pollution, etc., to reduce pollution, reduce pollution rate, and be beneficial to The effect of positioning germination

Inactive Publication Date: 2019-01-29
张坤
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] This method and the derived method all adhere the separated tissue pieces to the center of the test tube culture medium, and the tissue pieces are easy to move during the moving process, which increases the chance of contamination
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  • Method for isolating edible fungus strains

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Embodiment Construction

[0012] The method of this embodiment is as follows: after the sharp-nose tweezers and hands are disinfected, use tweezers to clamp the fruit body and rotate it evenly on the flame of an alcohol lamp to remove floating dust and some miscellaneous bacteria on the surface. Then hold the fruiting body vertically to reveal fresh sterile tissue. Burn the front end of the pointed tweezers 2cm in flames and wait for it to cool thoroughly. On the surface of the sterile tissue, use a sharp tip to scratch the size of a corn kernel, and finally, insert it obliquely into the tissue. Break off with tip. The test tube is inclined side down, such as figure 1 Shown in 2. Pull out the tampon with the little finger of the right hand. After the tweezers go off fire, clamp the tissue block, lightly burn it back and forth on the flame 3 times, put it into the test tube, and put the tampon on. Finally, the test tube is tilted, the separation medium faces downward, and the tissue block is shaken ge...

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Abstract

The invention relates to a method for isolating edible fungus strains. The method comprises the following steps: performing exposure of a fresh aseptic tissue of a fruiting body, cutting off a block from the surface of the aseptic tissue with a sharp tip, inserting the shape tip obliquely into the tissue, performing isolation with the shape tip, allowing a slope in a test tube to be downward, pulling out a tampon, clamping the tissue block with tweezers, burning the tissue block over flame lightly and to and fro, putting the burned tissue block into the test tube, covering the test tube with the tampon, tilting the test tube finally, allowing an isolation culture medium to face downwards, shaking the tissue block gently so as to make the tissue block slide directly from the mouth of the test tube to the bottom of the test tube containing the culture medium and be stuck between the culture medium and the test tube, and then putting the test tube at a temperature of 22-28 DEG C, whereingermination of hyphae occurs in 3 days, and transfer of the strains can be conducted in 8-12 days. According to the isolating method, operation can be conducted in an open environment without specialequipment, and in addition, alcohol and other disinfectants are not needed for the surface treatment, and direct clamping with the tweezers and even burning over the flame of an alcohol lamp are conducted, so that the surface dust and hybrid bacteria are burned by the flame; the tissue block does not pass over the culture medium, so that the contamination rate is reduced greatly.

Description

technical field [0001] The invention relates to a method for germination and separation of edible fungus strains by inoculating block blocks with culture medium upside down, which belongs to the separation method of microbial strains. Background technique [0002] Strain isolation is the operation method of transferring the local tissues, spores or basal hyphae of valuable fruiting bodies to the slant test tube culture medium to obtain pure cultured mycelia. There are two ways to obtain fungal resources: field collection and cultivation site collection, among which the collection of field specimens is mainly applicable to the domestication of wild species of edible fungi, genetic breeding, physiological ecology, and the isolation of various first-class species for research. And in the separation of the first-level species in the cultivation of edible fungi, the source of its seed mushrooms is mainly obtained from the cultivation field through seed retention. [0003] Edible...

Claims

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Application Information

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IPC IPC(8): A01G18/30
CPCA01G18/30
Inventor 张坤
Owner 张坤
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