Mink activin b protein and its preparation and application

Abstract

The invention relates to the field of molecular biology, in particular to a mink activin B protein and its preparation and application. The activin B protein is composed of two mink activin βB subunits, the gene sequence of which is obtained from mink animals through molecular biology techniques, and then expressed and purified in vitro with biologically active activin B protein. There are relatively few studies on activin B protein in mink animals. The present invention has isolated its gene sequence and improved the expression method of its high-purity protein. Research and application prospects of protein B.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a mink activin B protein and its preparation and application. Background technique [0002] Activin (Actvin, ACT), also known as activin, is a glycoprotein hormone firstly isolated and purified from porcine follicular fluid by Vale et al. and Ling et al. It is named after the inhibition of pituitary cell secretion of follicle stimulating hormone (Follicle Stimulating Hormone, FSH), which belongs to the transforming growth factor β superfamily member. Activin is mainly synthesized and secreted by ovarian granulosa cells and placenta. Because it has the biological activity of specifically promoting the secretion and synthesis of follicle stimulating hormone by pituitary cells, it is named activin. [0003] Both ACT and INH are dimers composed of two subunits. ACT is composed of β subunits, including homodimer ACTA (βA-βA) and ACTB (βB-βB) or heterodimer ACTAB (βA-βB). The three ...

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Problems solved by technology

At present, there is a large amount of domestic mink breeding, but the breeding level is low, especially the reproductive performance of mink, not only the litter size is small, but the living rate is also low

[0005] However, there are still few studies on mink ACTB in China at present, and there is no report on mink ACTB protein and cDNA sequences. research will create obstacles

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