Mustula Vison activin B protein, preparation and applications thereof

Abstract

The invention relates to the field of molecular biology, particularly to a Mustula Vison activin B protein, a preparation and applications thereof. According to the present invention, the activin B protein comprises two Mustula Vison activin betaB subunits; the gene sequence of the activin B protein is obtained from Mustula Vison animal through a molecular biological technology, and is expressed in vitro and purified to obtain the activin B protein with biological activity; in the prior art, the research of the activin B protein in Mustula Vison animals is less; and the gene sequence of the activin B protein is separated, and the high purity protein expression method is improved, such that the research basis and new ideas can be provided for the related research, and the research and application prospects of activin B protein are substantially improved.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a mink activin B protein and its preparation and application. Background technique [0002] Activin (Actvin, ACT), also known as activin, is a glycoprotein hormone firstly isolated and purified from porcine follicular fluid by Vale et al. and Ling et al. It is named after the inhibition of pituitary cell secretion of follicle stimulating hormone (Follicle Stimulating Hormone, FSH), which belongs to the transforming growth factor β superfamily member. Activin is mainly synthesized and secreted by ovarian granulosa cells and placenta. Because it has the biological activity of specifically promoting the secretion and synthesis of follicle stimulating hormone by pituitary cells, it is named activin. [0003] Both ACT and INH are dimers composed of two subunits. ACT is composed of β subunits, including homodimer ACTA (βA-βA) and ACTB (βB-βB) or heterodimer ACTAB (βA-βB). The three ...

AI Technical Summary

Problems solved by technology

At present, there is a large amount of domestic mink breeding, but the breeding level is low, especially the reproductive performance of mink, not only the litter size is small, but the living rate is also low

[0005] However, there are still few studies on mink ACTB in China at present, and there is no report on mink ACTB protein and cDNA sequences. research will create obstacles

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