Method for producing polysaccharide of Niudali by adopting culture medium reutilizing
A production method, Niu Dali's technology, which is applied in the field of Niu Dali polysaccharide production, can solve the problem that the output of wild Spatholobus spatholobus plants cannot meet the market demand, etc.
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Embodiment 1
[0059] A method for producing bovine herbaceous polysaccharide reutilized by culture medium, comprising:
[0060] Step 1) First, select the soon-to-mature seeds of Spatholobus Spatholobus, then sterilize the immature seeds, and then treat them at a temperature of 0° C. for 24 hours, and finally place them in a germination liquid medium for germination and culture until they grow 1 mm-long shoots. leaves, the soon-to-be-ripe seeds are the seeds of the Millipede spatholobus plant 90 days after flowering, and the disinfection includes: first cleaning the soon-to-mature seeds several times with a solution containing a mass volume concentration of 1g / mL calcium hypochlorite, and then The washed seeds were treated at a temperature of 70° C. for 20 seconds. The germination liquid medium contained: 1 / 4MS, 7 g / L sucrose and 0.1 mg / L 6-BA, and its initial pH value was 5.8.
[0061] Step 2) select the tender leaves grown through germination and culture as explants, place the explants on ...
Embodiment 2
[0075] A method for producing bovine herbaceous polysaccharide reutilized by culture medium, comprising:
[0076] Step 1) First, select the soon-to-mature seeds of Mildew Spatholobus, then sterilize the immature seeds, then treat them at a temperature of 2°C for 48 hours, and finally place them in a germination liquid medium for germination and culture until they grow 10mm-long shoots. leaves, the soon-to-be-ripe seeds are the seeds of the Millipede spathifolia plant 130 days after flowering, and the disinfection includes: firstly, using a solution containing mass volume concentration 5g / mL calcium hypochlorite to clean the soon-to-be-ripe seeds for several times, and then Treat the cleaned seeds at a temperature of 80° C. for 30 seconds. The method for obtaining the germination liquid medium is as follows: take the proliferation liquid medium used in step 5) in other embodiments, and collect the filtrate by filtering with a 4-mesh filter sieve , and then adjust the pH of the ...
Embodiment 3
[0091] A method for producing bovine herbaceous polysaccharide reutilized by culture medium, comprising:
[0092] Step 1) First, select the soon-to-mature seeds of Mildew Spatholobus, then sterilize the immature seeds, and then treat them at a temperature of 1°C for 36 hours, and finally place them in a germination liquid medium for germination and culture until they grow 5mm-long shoots. leaves, the soon-to-be-mature seeds are the seeds of the plant of Miltonia spaghetti 110 days after flowering, and the disinfection includes: firstly, using a solution containing mass volume concentration 3g / mL calcium hypochlorite to clean the soon-to-mature seeds for several times, and then Treat the cleaned seeds at a temperature of 75° C. for 25 seconds. The method for obtaining the germination liquid medium is: take the starting liquid medium once used in step 4) in other embodiments and use it in step 5). The final proliferation liquid culture medium was filtered with a 6-mesh filter si...
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