Composite sweetening agent

A compound sweetener and concentration technology, applied in the field of food additives, can solve the problems of low calorie, poor solubility and high cost, and achieve the effects of reducing production cost, good water solubility and simple method

Inactive Publication Date: 2019-03-01
SICHUAN INGIA BIOSYNTHETIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although stevioside and rebaudioside A have low calories and high sweetness, they also have a bitter aftertaste other than sweetness, and their taste and flavor cannot be compared with sucrose
Although rebaudioside D tastes similar to sucrose, its solubility is not good
And at present, although there are some sweetener compositions, their preparation methods are obtained by compounding relatively pure raw materials. This method has high requirements on the purity of raw materials, and the raw materials need to be purified before compounding, and the cost is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of recombinant microorganisms:

[0027] Rice (Oryza sativa) leaf total RNA was extracted and rice cDNA was obtained by reverse transcription. According to the EUGT11 gene sequence (Accession No. AK121682) in the GeneBank database, PCR amplification primers were designed, and the upstream and downstream primers were respectively introduced into the BamH I and Hind III sites, and the EUGT11 coding gene was obtained by PCR amplification.

[0028] Stevia total RNA was extracted and rice cDNA was obtained by reverse transcription. According to the EUGT11 gene sequence (Accession No. AK121682) in the GeneBank database, PCR amplification primers were designed, and the upstream and downstream primers were respectively introduced into the Nde I and Xho III sites, and the UGT76G1 coding gene was amplified by PCR.

[0029] The EUGT11 fragment and the expression vector pETDuet were double digested with BamH I and Hind III respectively, and the target fragment was re...

Embodiment 2～4

[0042] Using the method of Example 1, only part of the parameters during conversion were changed to obtain the product, wherein the changed parameters are shown in Table 2.

[0043] Table 2

[0044] Reaction parameters

Example 1

Example 2

Example 3

Example 4

OD600

100

80

120

110

Concentration of Rebaudioside A (g / L)

5

1

3

10

Concentration of trisodium citrate (mmol / L)

60

50

70

80

Zinc chloride concentration (mmol / L)

1

0.5

1.5

2

Sucrose concentration (%, w / v)

40

30

40

50

pH value

8.0

8.5

7.5

8.0

Reaction temperature (°C)

37

35

38

40

Reaction time (h)

24

20

28

30

[0045] Measure the amount of RD and RM in the products prepared in Examples 2-4, and record the weight ratio of RD and RM. The results are shown in Table 3.

[0046] table 3

[0047] Example number

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Abstract

The invention relates to a composite sweetening agent, belongs to the technical field of food additives, and solves the technical problem of providing the composite sweetening agent being good in mouth feel. The sweetening agent is synthesized through whole-cell catalysis, rebaudioside-A is used as a substrate, recombinant microorganisms are used as a catalyst, and in the presence of cane sugar, zinc chloride and trisodium citrate, the substrate is catalyzed for a reaction, so that a sweetening agent composition is obtained. A whole cell catalytic transformation method is adopted, the method is simple, and the transformation condition is controlled, so that the composition containing rebaudioside-D and rebaudioside-M in a specific compounding ratio can be obtained, and the production costcan be reduced. The mouth feel of the composite sweetening agent is similar to that of the cane sugar, any artificially-synthesized components are not introduced, and the composite sweetening agent ispurely natural, free from energy, and good in water solubility.

Description

technical field [0001] The invention relates to a compound sweetener, which belongs to the technical field of food additives. Background technique [0002] Sweeteners are substances that impart sweetness to soft drinks. At present, the most used natural sweetener is sucrose, which can provide people with a sweet taste, and its taste is generally accepted by people. However, sucrose has high calories, and diabetics and obese people need to be strict in their diets. Control, therefore, requires non-caloric or low-calorie sweeteners that replace sucrose. [0003] Steviol glycosides is a class of steviol glycosides extracted from the leaves of Stevia rehaudiana, which has high sweetness (250-300 times that of sucrose), low calorie (1 / 300 of sucrose) ), non-toxic and side effects, no carcinogens, food safety and other advantages, has been widely valued by the scientific community, industry and other fields, and has become the third development value and health respected after s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L27/30
CPCA23L27/36
Inventor 王勇吕华军孙雨伟李建华裴亮华君寇彦杰
Owner SICHUAN INGIA BIOSYNTHETIC CO LTD
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