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Serum antibody detection precision proficiency testing method, kit and system

A technology for serum antibodies and proficiency testing, which is used in biological testing, material testing products, measuring devices, etc.

Inactive Publication Date: 2019-03-19
SHANGHAI BLOOD CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to accurately evaluate the ability in this area, the ideal method is to prepare a set of antibody proficiency tests from strong to weak in constant dilution to examine the accuracy and accuracy of laboratory testing in the entire sequence from strong agglutination to weak agglutination. Its ability to discriminate subtle differences in agglutination, but such proficiency testing samples have not been reported so far

Method used

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  • Serum antibody detection precision proficiency testing method, kit and system
  • Serum antibody detection precision proficiency testing method, kit and system
  • Serum antibody detection precision proficiency testing method, kit and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Serum Antibody Detection Accuracy Proficiency Verification Method

[0043] see figure 1 , figure 1 It is a flow chart of the method for proficiency verification of serum antibody detection accuracy of the present invention, comprising the following steps:

[0044] a. The evaluator prepares the antibody serum sample and instructions; the antibody serum sample is a group of 5-20 tubes of antibody serum with different concentrations whose titer is between relatively weak positive and strong positive, and the concentration difference is the evaluator’s Known; said instructions have recorded the method of serum antibody detection accuracy proficiency test sample.

[0045] b. The evaluator shuffles the sequence of antibody serum samples according to predetermined rules, and packs them into small packages and delivers them to the subjects together with the instructions.

[0046] c. Subjects test the titer of antibody serum, rank them in a gradient from strong to wea...

Embodiment 2

[0049] Embodiment 2 Serum Antibody Detection Accuracy Application Example

[0050] For the antibody with a titer of 128, use AB serum as the diluent, and serially dilute 12 tubes of antibodies at a dilution rate of 1.5 times. The antibody titers from the first tube to the 12th tube are 85, 57, 38, 25, and 17 in sequence. , 11, 7.5, 5.0, 3.3, 2.2, 1.5, 0.98;

[0051] Shuffle the sequence of antibody serum diluted in fixed ratio according to predetermined rules, and distribute them in small packages to the application laboratory. The laboratory is required to distinguish the concentration of antibodies from strong to Weak gradient, compare the answer with the order of the antibody from strong to weak before the disruption, and judge whether it is consistent or how different it is;

[0052] For many laboratories participating in proficiency testing, this proficiency testing product is also divided into many groups, each group has different antibody sequence scrambling rules, and...

Embodiment 3

[0056] Example 3 Serum Antibody Detection Accuracy Proficiency Verification Kit

[0057] A serum antibody detection accuracy proficiency testing kit, comprising:

[0058] Antibody serum sample: the antibody serum sample is a high-titer antibody quantitatively diluted into a group of 5 to 20 tubes of antibody serum with different concentrations between relatively weak positive and strong positive, and the ratio coefficient is 0.5 to 0.95, and The difference in antibody concentration in each group is known to the evaluator;

[0059] Instructions: The instructions describe the method for proficiency verification samples for detection of serum antibodies.

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Abstract

The invention relates to the technical field of proficiency testing and specifically relates to a serum antibody detection precision proficiency testing method, kit and system. The serum antibody detection precision proficiency testing method comprises the following steps: preparing an antibody serum sample and a specification by an estimator; delivering the antibody serum sample and the specification to a subject by the estimator; detecting the valence of antibody serum by the subject, and delivering a result to the estimator; and carrying out comprehensive judgment by the estimator, and estimating the serum antibody detection precision proficiency of the subject. The kit comprises the antibody serum sample and the specification. The system comprises the kit, an estimation terminal, a subject terminal and a verification platform. The serum antibody detection precision proficiency testing method, kit and system are beneficial to objective and accurate estimation of serum antibody detection precision proficiency of laboratories or personnel relevant to immunohematology.

Description

technical field [0001] The invention relates to the technical field of proficiency verification, in particular to a method, kit and system for proficiency verification of serum antibody detection accuracy. Background technique [0002] Antibody exists in human and animal blood, interstitial fluid and exocrine fluid, and can specifically bind to the antigen structure. for the functional area. Antibody consists of two parts, heavy chain (Heavychain, Hchain) and light chain (Lightchain, Lchain). The heavy chain has four functional regions, one of which is in the variable region, also known as VH, and the rest are in the constant region including CH 1 , CH 2 , CH 3 . The light chain has two functional domains, the VL of the variable domain and the CL of the constant domain. Among them, VH and VL are the sites where antibody molecules bind to antigens due to their higher antigen-binding sites. CH 1 where is the genetic marker; CH 2 It is the complement binding site of an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 向东金沙沈伟
Owner SHANGHAI BLOOD CENT
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