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Bacillus subtilis GUMT323 and application thereof

A technology of Bacillus subtilis and tobacco black shank, which is applied in the fields of application, bacteria, and fungicides, and can solve the problems of pesticide residue environment, affecting farmers' income, and increasing the amount of pesticides used in the field.

Active Publication Date: 2019-03-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, capsicum anthracnose is one of the main diseases in capsicum production, which can cause damage throughout the growth period of capsicum, causing great losses
Guizhou Province is one of the main cultivation areas of pepper in my country. The long-term continuous cropping of pepper has caused the continuous expansion of the incidence of anthracnose, seriously affecting the income of farmers
At present, chemical pesticides are mainly used to prevent and control diseases in production. The resistance of pathogenic bacteria is increasing year by year, and the amount of pesticides used in the field is also increasing accordingly, which can easily cause pesticide residues and environmental pollution.

Method used

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  • Bacillus subtilis GUMT323 and application thereof
  • Bacillus subtilis GUMT323 and application thereof
  • Bacillus subtilis GUMT323 and application thereof

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Experimental program
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Embodiment Construction

[0014] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0015] 1. Isolation and screening of Bacillus subtilis GUMT323

[0016] 1. Materials and methods

[0017] 1.1 Materials

[0018] From 2017 to 2018, the plots with severe tobacco black shank disease were selected from Meitan County, Zunyi City, Guizhou Province, and the rhizosphere soil of healthy tobacco plants was collected. The 5-point sampling method was used to mix soil samples from the same plot to count as 1 soil sample. Soil samples were collected in sterile ziplock bags, and soil collection tools were sterilized with 75% alcohol to avoid soil microbial mixing between different fields.

[0019] 1.2 Medium

[0020] LB medium is used for the isolation and preservation of Bacillus; PDA medium is used for plate confrontation culture experiments; protease detection medium, cellulase detection medium, siderophilic detection medium, phosphatase detection medi...

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Abstract

The invention discloses bacillus subtilis GUMT323, which is collected in the China Center for Type Culture Collection, with the collection number of CCTCC NO: M 2018873. The invention further discloses application of the bacillus subtilis GUMT323 to control of tobacco black shank. The bacillus subtilis GUMT323 has an obvious ability of antagonizing both phytophthora nicotianae and the ability of antagonizing pepper anthracnose.

Description

technical field [0001] The invention relates to a bacillus subtilis GUMT323 and an application thereof, belonging to the technical field of biological control. Background technique [0002] Tobacco black shank (Phytophthora nicotianae) is one of the most destructive soil-borne diseases in tobacco production in Guizhou. The economic loss caused by this disease is nearly ten million yuan every year, seriously affecting the economic income of tobacco farmers. At present, chemical control is mostly used in production, but the main chemical has a long service life, the resistance of pathogenic bacteria is increasing year by year, and the negative effects of pesticide residues and environmental pollution are also gradually increasing. Therefore, the use of biological control to control tobacco diseases is a hot research field at present. [0003] Bacillus (Bacillus spp.) is currently the most commonly used microbial species in biological control research. Bacillus has the prospec...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C12R1/125
CPCA01N63/00C12R2001/125C12N1/205
Inventor 丁海霞彭丽娟余水谢鑫
Owner GUIZHOU UNIV
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