A assay kit for serum amyloid a
An amyloid and serum starch technology, applied in the field of medical biological detection, can solve the problems of undisclosed accuracy, precision and linearity, lack of calibrators and quality control materials, unfavorable clinical promotion and use, etc., and achieve accurate detection results. Reliable, accurate and linear results
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Embodiment 1
[0039] Example 1: Preparation of Anti-human Amyloid A Antibody Latex Particles
[0040] (1) Preparation of anti-human serum amyloid A antibody solution: take the anti-human serum amyloid A antibody and dissolve it in Tris-HCl buffer solution with a pH of 6.8 to prepare an anti-human serum amyloid with a concentration of 0.5 mg / mL Protein A antibody solution;
[0041] (2) Preparation of latex microsphere suspension: According to the ratio of mass ratio of 1:3, two kinds of glycidyl methacrylate latex particles with particle diameters of 50-70nm and 100-200nm were dissolved in Tris-HCl buffer solution, the concentration was 1% (w / v), then sequentially added polyaspartic acid with a concentration of 15mg / mL and N,N-methylenebisacrylamide at a concentration of 13mg / mL, and incubated at room temperature for 45 minutes at high speed Centrifuge (12000rpm, 30min), discard the supernatant, wash the latex microspheres twice with the same volume of Tris-HCl buffer to remove excess cross...
Embodiment 2
[0043] Example 2: Preparation of Anti-human Amyloid A Antibody Latex Particles
[0044] Compared with Example 1, only the ratio of anti-human serum amyloid A antibody solution and latex microsphere suspension in step (3) is different, and the volume ratio in this example is 1:2.
Embodiment 3
[0045] Example 3: Preparation of Anti-human Amyloid A Antibody Latex Particles
[0046] Compared with Example 1, only the ratio of anti-human serum amyloid A antibody solution and latex microsphere suspension in step (3) is different, and the volume ratio in this example is 1:3.
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