Linear rapid dual-temperature PCR amplification automatic control device and control method

Abstract

The invention discloses a linear rapid dual-temperature PCR amplification automatic control device and a control method. A linear transfer transport module is installed on a multi-tank water bath pot,a control circuit board is connected to the transfer transport module and an upper computer module, and a sample container fixing disc is installed on the transfer transport module; a visualized fluorescence detection device is adsorbed onto the outer wall of the multi-tank water bath pot by a magnet piece, a sample container comprises a container used for installing a PCR eight-connected tube, aPCR capillary tube, a capillary pipet and a centrifuge tube; and two thermostatic sample pools are respectively arranged corresponding to a denaturation phase and an extension phase, the transfer transport module controls the sample container of the sample container fixing disc to circularly transfer back and forth and to stay in the thermostatic sample pools in a denaturation phase and an extension phase, and multiple circulation in two temperature zones is performed. The device realizes rapid double-temperature PCR amplification of multiple samples and multiple circulation, greatly shortensthe detection time, and is low in production cost, convenient to install, high in universality, small in size and easy to carry.

Description

technical field [0001] The invention relates to a PCR amplification reaction device and method in the field of rapid detection of food safety, in particular to a linear rapid dual-temperature PCR amplification automatic control device and control method. Background technique [0002] Polymerase Chain Reaction (PCR) is a technique widely used in molecular biology to rapidly amplify DNA fragments in vitro, and is one of the important methods for detection in industries such as food safety, clinical diagnosis, and quarantine inspection. The standard PCR process is divided into three steps: (1) denaturation (90°C-96°C), the hydrogen bond of the double-stranded DNA template is broken under the action of heat to form single-stranded DNA; (2) annealing (60°C-65°C), the system When the temperature is lowered, the primer will combine with the DNA template to form a partial double strand; (3) extension (70°C-75°C), under the action of DNA polymerase, using dNTP as raw material, starti...

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Problems solved by technology

[0004] In summary, although existing commercial PCR instruments can achieve the purpose of automatic amplification, they are often expensive and have poor compatibility with sample containers, making it difficult to popularize and apply them on a large scale. Increasingly demanding

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