Anti-interference electrochemical detection sensor and manufacturing method therefor

Abstract

The invention relates to an anti-interference electrochemical detection sensor and a manufacturing method therefor. The sensor has a reaction cell and an injection flow channel, and the injection flowchannel is connected to the reaction cell. A reaction electrode and a reaction reagent layer disposed on the detection electrode for reacting with the target detection object are disposed in the reaction cell, and the reaction reagent layer is exposed in the reaction cell. An auxiliary electrode is disposed in the injection flow channel, and the interior and / or surface of the auxiliary electrodecontains an anti-interference substance capable of reacting with an interference substance in the sample to be tested. The anti-interference electrochemical detection sensor further has an electrode connector for connecting to an external detecting device, and the electrode connector is electrically connected to the detection electrode. The anti-interference electrochemical detection sensor is provided with an auxiliary electrode in the injection flow channel in the front stage of the reaction cell, and the auxiliary electrode contains an anti-interference substance capable of reacting with the interference substance. Therefore, during the detection, the interference substances in the sample solution can be removed, the influence of the interference substances on the detection results is eliminated, and the accuracy and reliability of the detection results are ensured.

Description

Technical field [0001] The invention relates to the field of biochemical detection, in particular to an anti-interference electrochemical detection sensor and a manufacturing method thereof. Background technique [0002] In recent years, the detection of blood glucose, uric acid, lactic acid and other substances has attracted more and more attention. The detection methods mainly include gravimetric method, titration method, phosphotungstic acid reduction method, high performance liquid chromatography, enzyme-linked photometry, enzyme-linked colorimetry and electrochemical method. [0003] The gravimetric method and titration method not only have a long operation time, but also have relatively large errors and low accuracy. The phosphotungstic acid reduction method has poor specificity for detecting uric acid, and the ascorbic acid contained in the blood sample has a relatively large interference to it, and the test error is large. High-performance liquid chromatography has high s...

AI Technical Summary

Problems solved by technology

[0003] The gravimetric method and titration method not only take a long time to operate, but also have relatively large errors and low accuracy

The phosphotungstic acid reduction method has poor specificity for the detection of uric acid, and the ascorbic acid contained in the blood sample has a relatively large interference with it, resulting in a large test error

High-performance liquid chromatography has relatively high sensitivity, but it needs large-scale instruments and special devices for detection. The test requires a large amount of blood, takes a long time, and is expensive. It is only suitable for hospitals, and it is not convenient for patients to use at home.

Clinically, enzyme-linked colorimetry and enzyme-linked photometry are often used to measure the concentration of uric acid in body fluids, but they have high requirements for instruments, professional operators and special reagents, and are usually affected by enzyme activity, so they are not suitable for home use.

Electrochemical detection can be used in home and other occasions, but there are often other interfering factors in blood waiting samples besides the target substance to be tested, especially the presence of interfering substances such as ascorbic acid, which affects the corresponding test results

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