Islet cell cryopreservation solution and use method thereof

A technology for cryopreservation and islet cells, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problem of inability to meet islet transportation and other problems, achieve short-term and long-distance transportation, increase cryopreservation time, and reduce fragmentation. Effect

Active Publication Date: 2019-04-16
HUNAN XENO LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the longest time for islet cryopreservation and transportation reported internationally is only 72 hours, which is far from meeting the global islet transportation (≥4 days), and the research object is only mouse islets (Yasuko et al., 2013)

Method used

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  • Islet cell cryopreservation solution and use method thereof
  • Islet cell cryopreservation solution and use method thereof
  • Islet cell cryopreservation solution and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1, low temperature preservation solution and preservation method test of the present invention

[0045] The present invention is aimed at the islet cells of newborn pigs and young pigs that have been separated and purified. Dimethylsulfoxide 1%, 4-Hydroxyethylpiperazineethanesulfonic acid 5mM, SD-282 1uM, 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) 5mM, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (Pefabloc) 0.5mM, pre-frozen at -20°C and stored in a tube at a concentration of 5000-10000IEQ / 1-5mL of porcine islet cells Store at 4°C, recover the cell counts and perform relevant tests on day 1, day 3, and day 5.

Embodiment 2

[0047] The present invention is aimed at the islet cells of newborn pigs and young pigs that have been separated and purified. Dimethylsulfoxide 1%, 4-Hydroxyethylpiperazineethanesulfonic acid 5mM, SD-282 1uM, 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) 5mM, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (Pefabloc) 0.5mM, pre-frozen at -20°C and stored in a tube at a concentration of 5000-10000IEQ / 1-5mL of porcine islet cells Store at 4°C, recover the cell counts and perform relevant tests on day 1, day 3, and day 5.

Embodiment 3

[0049] The present invention is aimed at the islet cells of newborn pigs and young pigs that have been separated and purified. Dimethylsulfoxide 10%, 4-Hydroxyethylpiperazineethanesulfonic acid 5mM, SD-282 1uM, 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) 5mM, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (Pefabloc) 0.5mM, pre-frozen at -20°C and stored in a tube at a concentration of 5000-10000IEQ / 1-5mL of porcine islet cells Store at 4°C, recover the cell counts and perform relevant tests on day 1, day 3, and day 5.

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Abstract

The invention discloses an islet cell cryopreservation solution and a using method thereof. The cryopreservation solution is prepared by adding 10-40% of a conditioned medium of mesenchymal stem cells, 10-50% of human blood albumin, 1-10 mM of gamma-aminoethanesulfonic acid, 0.1-10% of dimethyl sulfoxide, 1-20 mM of 4-hydroxyethylpiperazineethanesulfonic acid, 21nM-50uM of SD-28, 1-20 mM of 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid and 0.1-1.0 mM of 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride in a minimal medium. The cryopreservation solution can prolong the cryopreservation time without affecting various functional indexes of islet cells so as to meet the short-term long-distance transportation of the islet cells.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation of animal islet cells, and in particular relates to a liquid for cryopreservation of islet cells and a method for using the same. Background technique [0002] In 2017, the International Diabetes Federation (IDF) released the eighth edition of the global diabetes map. The new version of the map shows that there are currently 425 million diabetics in the world, and it is estimated that by 2045, there will be nearly 700 million diabetics. Although the intensive blood sugar control program can control the occurrence of hyperglycemia in diabetes, it cannot prevent the occurrence of hypoglycemia, nor can it prevent the occurrence of diabetic complications. Patients with end-stage diabetes need to spend a huge amount of medical treatment due to the complications of multiple organ damage. 20% of patients with diabetes complications cannot afford huge medical expenses and die because of giving u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 王维王佳谷星石李桑徐畅
Owner HUNAN XENO LIFE SCI
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