A kind of enzyme composition and its application in cosmetics with whitening function
A composition and whitening technology, which is applied in the direction of cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of poor stability, slow onset of enzyme cosmetics, and inability to exert enzyme functions normally, and achieve unique texture, easier absorption, Good product stability
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Embodiment 1-7
[0038] The formula is shown in Table 1.
[0039]
[0040] The method of the essence, specifically
[0041] (1) mixing and stirring the rice fermentation product filtrate, yeast polypeptide, membrane-covered yeast fermentation product, snow lotus fermentation liquid and medicinal rhubarb extract at 25-35° C. to prepare an enzyme composition;
[0042] (2) Mix 1,2-pentanediol, 1,3-propanediol, betaine, trehalose, methyl gluceth-10, sodium polyglutamate, sodium hyaluronate, tremella Mix the solid extract, disodium edetate and purified water, heat to 85-95°C, stir to dissolve, continue to keep warm and stir for 15-25min, then cool down to 42-48°C, and then add the formulated enzyme combination in sequence substance, tranexamic acid, rose water, nicotinamide, β-glucan, carboxymethyl chitosan, acetyl hydroxyproline, nano-encapsulated ceramide, preservatives, p-hydroxyacetophenone, stirring and cooling to 36 Filter at -40°C to obtain the essence.
experiment example 1
[0059] Experimental Example 1 Whitening Effect Experiment
[0060] 1. Experimental population
[0061] A total of 275 female volunteers aged 20-50 were selected and randomly divided into 11 groups with 25 people in each group.
[0062] 2. Experimental equipment
[0063] German CK multi-probe skin test system MPA9, melanin and hemoglobin test probe (MexamterMX18).
[0064] 3. Experimental method
[0065] After cleansing the face of the subjects every morning and evening, take 0.04ml (1ml=50 drops, that is, 2 drops can be used) the whitening essence prepared in this embodiment and the comparative example and apply it on the face, gently massage until absorbed, and use it continuously for 28 days;
[0066] Before the test, the subjects need to clean their face with clean water, wipe it clean with a facial tissue, and sit quietly in a constant temperature and humidity laboratory (temperature 20±1°C, humidity 55±3) for 30 minutes before starting the measurement. Select the 4*4c...
experiment example 2
[0074] Experimental example 2 antibacterial performance test
[0075] Take 0.5g of the whitening essence prepared in Example 1-5 and Comparative Example 4-5, add 10ml of 55wt% ethanol solution, stir and dissolve to obtain a sample solution, and save it for future use.
[0076] Preparation of test bacterial suspension: Inoculate Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa and Aspergillus niger in the test tube culture medium, and then add Aseptically treated distilled water was shaken to obtain a suspension of experimental bacteria. Take 15ml of melted SOB medium and place it in a sterilized plate, then add 1ml of the experimental bacteria dropwise and make it spread to the bottom of the plate. After the culture medium is solidified, put 4 Oxford cups on each plate, then add 0.1ml of plant-derived bacteriostatic agent solution, and measure the diameter of the inhibition zone after culturing at 37°C for 24 hours (unit: mm...
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