Magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides

A hydrophilic material, zwitterion technology, applied in the field of biochemical analysis, can solve the problems of increasing the cost of analysis methods, complex synthesis procedures, long incubation time, etc., and achieves strong binding ability, high enrichment selectivity, and good recovery rate. Effect

Active Publication Date: 2019-08-16
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

[0005] However, the synthesis of these hydrophilic materials usually requires complex synthetic procedures, multi-step functionalization, and long incubation times
This tedious process greatly increases the cost of analytical methods, greatly limiting their application in real samples

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  • Magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides
  • Magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides
  • Magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides

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Embodiment Construction

[0059] In order to make the technical solution of the present invention clearer, the present invention is further described below, and any solution obtained by equivalent replacement and conventional reasoning of the technical features of the technical solution of the present invention falls within the protection scope of the present invention.

[0060] A magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides using hydroxyapatite nanofibers as a carrier to immobilize Fe 3 o 4 Nanoparticles and Au nanoparticles, through the thiol group in the zwitterionic tripeptide L-glutathione interacting with Au and Fe 3 o 4 The affinity between the surface modification, the formation of mHN / Au-GSH nanofibers.

[0061] Further, the preparation method of mHN / Au-GSH nanofibers is as follows:

[0062] S1: Disperse 2.0g of hydroxyapatite nanofibers and 0.75g of iron acetylacetonate in 400mL of triethylene glycol to obtain a mixture; ...

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Abstract

The invention provides a magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides. The material is used for glycopeptide enrichment and identification. One-dimensional hydroxyapatite nanofibers (HN) are used as carriers to fix Fe3O4 nanoparticles and Au nanoparticles, and surface modification is performed by the affinity interaction betweenthiol groups in zwitterionic tripeptide L-glutathione and Au and Fe3O4 to form mHN/Au-GSH nanofibers. The mHN/Au-GSH nanofibers have high detection sensitivity (2fmol), high recovery rate (89.65%), strong binding ability (100mg/g), and high enrichment selectivity (1:100) on glycopeptides.

Description

technical field [0001] The invention relates to a magnetic nanofiber-based zwitterionic hydrophilic material for selectively capturing and identifying glycopeptides, and belongs to the technical field of biochemical analysis. Background technique [0002] Proteins / peptides, carbohydrates, nucleic acids, etc., as essential and important biological components, mediate most of the basic biological processes, such as cell communication, immune response and enzymatic reaction. Detection and characterization of target biomacromolecules is a great challenge, especially for low-abundance analytes, due to complex composition and severe interferences. Therefore, sample preparation is an integral step in the analysis process. The development of affinity materials for the selective separation and preconcentration of target biomacromolecules is an effective way to reduce impurity interference and improve detection sensitivity. To date, a variety of materials have been developed, includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M11/49D06M11/83D06M13/402G01N1/34G01N1/40G01N33/68
CPCD06M11/49D06M11/83D06M13/402G01N1/34G01N1/4044G01N33/6851
Inventor 李洁郇伟伟王步川吴敏洁赵佳俊
Owner ZHEJIANG FORESTRY UNIVERSITY
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