Method to improve germination of Brasenia schreberi seeds and application of method
A seed and technology of water shield, applied in the field of improving seed germination of water shield, can solve the problems of low germination rate and the like
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Embodiment 1
[0025] 1. Seed collection: collect mature fruit clips, process the outer testa to obtain yellow-brown water shield seeds, and set aside.
[0026] 2. Seed pretreatment: soak the seeds selected in step 1 with 75% alcohol for 5 minutes, and rinse them with tap water. Then disinfect with 2% sodium hypochlorite solution for 20 minutes, and rinse with sterile water 3 times.
[0027] 3. Preparation of soaking solution: take gibberellin GA 3 Dilute to 1.0, 1.5, 2.0 and 2.5mmol / L with water respectively.
[0028] The pretreated seeds were subjected to the following two treatments:
[0029] 1) Gibberellin soaking treatment after cold-wet stratification
[0030] The pretreated Brasenia seeds were subjected to cold-wet stratification, wherein the seed: wet perlite volume ratio ≈ 1:3 and mixed well. Store in a refrigerator at 4°C for 0-240 days to ensure humidity. Afterwards, 50 treated seeds were taken as one portion, each group was repeated 4 times, soaked in 50mL of 0, 1.0, 1.5, 2....
Embodiment 2
[0041] 1. Seed collection: collect mature fruit clips, process the outer testa to obtain yellow-brown water shield seeds, and set aside.
[0042] 2. Seed pretreatment: soak the seeds selected in step 1 with 75% alcohol for 5 minutes, and rinse them with tap water. Then disinfect with 1:20 sodium hypochlorite solution for 20 minutes, and rinse with sterile water 3 times.
[0043] 3. Rapid dehydration of seeds with silica gel for 0, 1, 3, 6, 12, 18, 24, 30 and 36 hours, wherein the volume ratio of seeds to silica gel is ≈1:3 and the final water content is 24.0% and 18.1% respectively , 16.0%, 13.1%, 9.7%, 8.2%, 7.2%, 6.7% and 6.1%.
[0044] 4. The dehydrated seeds are subjected to cold-wet stratification treatment, in which the volume ratio of wet perlite is ≈1:3, mixed evenly, and stored in a refrigerator at 4°C for 0-135d to ensure humidity. The wet perlite in this embodiment is obtained by uniformly mixing 1 part by weight of perlite and 1.4-2 parts by weight of water.
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