Islet cell microcapsule for tissue engineering and preparation method thereof

An islet cell and tissue engineering technology, applied in the field of medical bioengineering, can solve the problems of poor mechanical properties, achieve the effect of improving mechanical properties, strong stability and mechanical strength, and inhibiting fibrosis

Active Publication Date: 2021-09-03
SHANDONG BRANDEN MEDICAL DEVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate-polylysine-sodium alginate (APA) microcapsules are prone to tissue fibrosis due to easy adsorption of proteins and cells, and their mechanical properties are poor

Method used

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  • Islet cell microcapsule for tissue engineering and preparation method thereof
  • Islet cell microcapsule for tissue engineering and preparation method thereof
  • Islet cell microcapsule for tissue engineering and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of Tissue Engineering Islet Cell Microcapsules

[0034] (1) Isolation and purification of SD rat islet cells

[0035]Islet digestion and isolation: select adult male SD rats, put them in a box and anesthetize with ether, fix the rats and continue to inhale ether for anesthesia, routinely disinfect the operation field, make a "V"-shaped incision on the abdomen from the reproductive part of the rat, fully The common bile duct was exposed, and the two ends of the duodenum were clamped with arterial clips at the place where the common bile duct enters the duodenum, and the rats were sacrificed by opening the chest and breaking the heart. Insert a 24G trocar into the common bile duct at the bifurcation of the left and right hepatic ducts. After success, slide the plastic needle into the common bile duct for about 2-4mm, fix the needle with tweezers to avoid sliding, and perfuse the collagenase V solution retrogradely into the pancreatic duct8 ~10ml (co...

Embodiment 2

[0039] Embodiment 2: The islet cells are placed in the sodium alginate solution modified by arginine-glycine-aspartic acid peptide with a mass percentage concentration of 15g / L and mixed to make a suspension, so that each milliliter of the suspension contains 1× 106 cells; using the InotechIE-50R Encapsulator microcapsule instrument, using the vibrating nozzle method, by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, the cell suspension was sprayed into the state of micro-droplets with a diameter of 150-400μm at a concentration of 80 In mmol / L calcium chloride solution, the volume ratio of cell suspension to calcium chloride solution is 1:100. React for 5 minutes to form calcium alginate gel beads. After the rubber beads settle, suck out the upper calcium chloride solution, then wash with 0.9% sodium chloride injection for 3 times, then put the rubber beads into the active amino solution with a concentration of 0....

Embodiment 3

[0040] Embodiment 3: The islet cells are placed in the sodium alginate solution modified by arginine-glycine-aspartic acid peptide with a mass percentage concentration of 25g / L and mixed to make a suspension, so that each milliliter of the suspension contains 6× 106 cells; using the InotechIE-50R Encapsulator microcapsule instrument, using the vibrating nozzle method, by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, the cell suspension is sprayed into the state of micro-droplets with a diameter of 700-1000μm at a concentration of 120mmol / L of calcium lactate solution, in which the volume ratio of cell suspension to calcium chloride solution is 1:100. React for 10 minutes to form calcium alginate beads. After the rubber beads settle, suck out the upper calcium chloride solution, then wash with 0.9% sodium chloride injection for 3 times, then put the rubber beads into the active amino solution with a concentration...

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Abstract

The invention belongs to the field of medical bioengineering, and relates to a pancreatic islet cell microcapsule for tissue engineering and a preparation method thereof. The present invention adopts the structure of sodium alginate-active amino component-polyethylene glycol, and its inner layer is wrapped with sodium alginate modified by arginine-glycine-aspartic acid peptide, and then hyperbranched polyethylene The imine-coupled polylysine was used as a stabilizer, and the hyperbranched polyethyleneimine cross-linked and reinforced the polylysine layer to improve the mechanical properties of the microcapsules. The outermost layer of the microcapsule is composed of a polyethylene glycol chain segment fixed on one side of the active amino component. Because of its sliding properties, it can inhibit the adhesion of proteins and cells. Since polyethylene glycol is a material that tends to be "invisible" in the human body, the microcapsules of the present invention can inhibit fibrosis to a certain extent, thereby prolonging the survival time of active cells. It can improve the blood sugar level of patients with type 1 diabetes to a certain extent.

Description

technical field [0001] The invention belongs to the field of medical bioengineering, and relates to a pancreatic islet cell microcapsule for tissue engineering and a preparation method thereof. Background technique [0002] The current treatment of type 1 diabetes mainly relies on insulin, but even with the optimal insulin dose, some patients still have unstable blood sugar control, which in turn causes microvascular and macrovascular complications, such as diabetic nephropathy, diabetic retinopathy and peripheral vascular disease, etc. , which can eventually lead to organ failure and death. [0003] In the late 1970s, Najarian, Suther, and Lajiader et al used portal vein and spleen methods to perform allogeneic islet cell (Isletcell, IC) transplantation for the treatment of non-autoimmune diabetes mellitus (DM). In 1990, Sharp et al. reported that allogeneic IC transplantation was used to treat patients with type 1 DM successfully, which should be attributed to the progres...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61L27/38A61L27/50A61L27/20A61L27/18
CPCA61L27/18A61L27/20A61L27/3804A61L27/50A61L2300/622A61L2400/06C08L71/02C08L5/04
Inventor张海军袁坤山王如蒙鲁手涛刘黎明曹文瑞张淑欣徐海荣
OwnerSHANDONG BRANDEN MEDICAL DEVICE