Early screening method of mango fruit color based on dual-fragment analysis

A screening method and double-fragment technology, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as identification difficulties and achieve the effect of convenient early selection

Pending Publication Date: 2019-11-05
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because of its long childhood, it has brought certain difficulties to the identification of varieties

Method used

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  • Early screening method of mango fruit color based on dual-fragment analysis
  • Early screening method of mango fruit color based on dual-fragment analysis

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Experimental program
Comparison scheme
Effect test

Embodiment

[0048] As attached to the experiment figure 1 Shown:

[0049] A method for early screening of mango fruit color based on double-segment analysis, characterized in that it comprises the following steps:

[0050] A. Extraction of sample DNA:

[0051] According to the requirements of sample DNA extraction, young and tender leaves are selected as test materials. The specific extraction method is as follows:

[0052] (1) Weigh 0.5-1.0 grams of mango leaves, quickly add liquid nitrogen and grind until the material is completely ground into powder, quickly transfer to a centrifuge tube, add 10ml of 65°C preheated extract 10ml (100mM Tris-HCl, 1.4 M NaCl, 20mM EDTA, 2% CTAB, 3% PVP, 0.5% ß-mercaptoethanol), mix thoroughly, lyse at 65°C for 60 minutes, shake once every 10 minutes;

[0053] (2) Add 10ml of chloroform / isoamyl alcohol (24:1), shake at room temperature for 10 minutes, and centrifuge at 4000rpm for 10 minutes;

[0054] (3) Take the supernatant, add an equal volume of c...

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Abstract

The invention relates to an early screening method of mango fruit color based on dual-fragment analysis. The early screening method is characterized by comprising the following steps of A, extractingDNA of samples; B, performing PCR amplification on the samples; C, recovering amplification fragments, and performing connecting and sequencing; and D, analyzing the fragments, and determining the color of fruits. The scheme has the following prominent substantive features and marked improvement that mango hybridizing or seedling progeny are long in juvenile phase, blossoming, fruit bearing and fruit color determining require time of 5-6 years; and through the adoption of the scheme, the fruit color of hybridizing or seedling progeny can be determined as soon as possible; after germination ofhybrid seedlings or sexual seedlings, leaf blade samples are analyzed, and the samples are easy to take; the scheme is easy and practicable to operate, detection can be finished within 4-5 days, and the identification period of the color of the mango fruits can be greatly shortened; the scheme is promoted to production of mangoes, so that early selection of the color of the mango fruits can be facilitated, and technical support is provided for directional breeding and parent matching of the color of the mango fruits.

Description

technical field [0001] The invention relates to the technical field of early identification of plant fruit color, in particular to an early screening method for mango fruit color based on double-segment analysis. Background technique [0002] Mango is one of the famous tropical fruits. Because of its delicate flesh and unique flavor, it is deeply loved by people. Therefore, it is known as the "King of Tropical Fruits" and is deeply loved by people. Mangoes are native to India and Malaysia. There are more than 70 countries producing mangoes in the world, 90% of which are concentrated in India, Pakistan, Bangladesh, Myanmar, Malaysia and other countries in Asia; eastern and western Africa, Tanzania, Zaire, and Brazil in the Americas China, Mexico, Florida and Hawaii in the United States are all cultivated. my country's mangoes are mainly distributed in Hainan, Guangdong, Yunnan, Guangxi, Fujian and Sichuan provinces, and are an important part of the local agricultural economic ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6806
CPCC12Q1/6895C12Q1/6806C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 赵志常高爱平黄建峰罗睿雄
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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