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Method for identifying authenticity of deer horn

A technology of authenticity and reindeer, which is applied in the field of authenticity identification of reindeer antlers, can solve the problems of inability to distinguish reindeer antlers from other antlers, etc., and achieves the effects of economical and reasonable inspection methods, improved accuracy, and cost savings.

Pending Publication Date: 2019-12-03
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that reindeer antlers cannot be effectively distinguished from other antlers in the prior art, this application discloses a method for authenticity identification of reindeer antlers, which can effectively distinguish reindeer antlers from other antlers

Method used

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  • Method for identifying authenticity of deer horn
  • Method for identifying authenticity of deer horn
  • Method for identifying authenticity of deer horn

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Experimental program
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Embodiment 1

[0028] 1 Instruments and materials

[0029] 1.1 Instrument

[0030] Thermo Ultimate 3000 ultra-high performance liquid chromatography connected in series with Thermo Scientific Fusion-Orbitrap high-resolution mass spectrometer; Thermo Hypersil GOLD column (100×2.1mm, 3μm)

[0031] 1.2 Materials

[0032] Sartorius-10K ultrafiltration tube (batch number: 170700SVS); trypsin (Sigma company, batch number: SLBG6452V); reindeer antler (5 batches), water antler (2 batches), moose antler (2 batches), roe deer antler (2 batches) , red antler (2 batches), sika antler (2 batches), the above samples are the samples kept in the herbarium of the unit or the samples collected from the market, and the reference medicinal materials of the red antler (China Institute for Food and Drug Control, referred to as the National Institute for Inspection; batch number: 121487- 200501); guanidine hydrochloride, tris(Tris), ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), iodoacetamide (IAA...

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Abstract

The invention relates to the technical field of traditional Chinese medicine identification and particularly relates to a method for identifying the authenticity of a deer horn. The method comprises astep of performing enzymolysis on a sample to be detected by using trypsin, and detecting by using an ultra-high-performance liquid chromatograph and a high-resolution mass spectrometer, a step of judging whether a mass spectrum peak exists or not by adopting a method of combining two modes of precise ions and relative retention time, so that the authenticity of the deer horn is judged, the accuracy of the detection method is improved, and the method is superior to a method adopting a single mode.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicines, in particular to a method for identifying the authenticity of reindeer horns. Background technique [0002] Reindeer horns are the horns of reindeer animals belonging to the genus Reindeer of the deer subfamily of deer. It has the effects of warming and nourishing the liver and kidney, reducing swelling and removing blood stasis. Pain and other symptoms. This product is widely used in China, but there has been a lack of effective authenticity detection methods. [0003] The current quality standards for reindeer antlers are recorded in the 2001 edition of "Heilongjiang Provincial Standards for Chinese Medicinal Materials". The standards only include descriptions of the appearance and properties of the whole antler, and there are no other valid inspection items. Because this product needs to be sliced, sawed into small pieces, split into small nails, groun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/7266
Inventor 王玉团邢晟杭宝建陈晓由鹏飞石峰
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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