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Moloney murine leukemia virus titer detection kit and titer detection method

A technology of murine leukemia virus and detection kit, which is applied in the field of virus molecular biology, can solve the problems of not being able to truly and accurately reflect the real infection activity of the virus, overestimating the virus titer, etc., and achieve the effect of rapid detection and high versatility

Inactive Publication Date: 2019-12-24
YUNZHOU BIOSCIENCES (GUANGZHOU) INC
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Problems solved by technology

However, the RT-qPCR method will also count the nucleic acid of virus particles that contain nucleic acid but have lost their infectious activity, which cannot truly and accurately reflect the true infectious activity of the virus, and will overestimate the titer of the virus, thus limiting the use of this method. application

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  • Moloney murine leukemia virus titer detection kit and titer detection method
  • Moloney murine leukemia virus titer detection kit and titer detection method
  • Moloney murine leukemia virus titer detection kit and titer detection method

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Embodiment Construction

[0025] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of ...

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Abstract

The invention discloses a Moloney murine leukemia virus titer detection kit and a titer detection method. The detection kit comprises a virus specific amplification primer pair, the virus specific amplification primer pair is used for amplifying a target virus segment on a packaging signal psi of a Moloney murine leukemia virus genome; therefore, whether the to-be-detected virus contains the fluorescent label is determined, the titer detection is not influenced, furthermore, viruses which are infected with cells and are successfully integrated are detected by a qPCR method, therefore, the Moloney murine leukemia virus titer detection kit and the titer detection method provided by the invention can reflect the real infection activity of the virus, are not influenced by the extraction efficiency of cell genome DNA, and have the advantages of rapid, accurate and convenient detection.

Description

technical field [0001] The invention relates to the field of virus molecular biology, in particular to a Moloney murine leukemia virus titer detection kit and a titer detection method. Background technique [0002] Moloney murine leukemia virus (MMLV) is a type of retrovirus, and its genome consists of two identical positive-strand RNAs. After the virus infects cells, its RNA genome can be reverse-transcribed into DNA to integrate into the host genome and be stably passaged. Therefore, MMLV can efficiently introduce the target gene (or RNAi) into primary cells or cell lines of mice or other animals. Due to this characteristic of MMLV, recombinant MMLV is widely used in fields such as gene therapy and tumor research. The production of recombinant MMLV in the laboratory is to co-transfect cells with expression vectors and auxiliary packaging plasmids, and complete virus packaging in cells. The packaged pseudovirion particles are secreted into the extracellular medium, and th...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
CPCC12Q1/6851C12Q1/702C12Q2600/166C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 蓝田施金秀
Owner YUNZHOU BIOSCIENCES (GUANGZHOU) INC
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