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The domestication process of tissue culture seedlings of a hard-to-root clone of Acacia blackwood

A technology for tissue culture seedlings and clones, which is applied in the field of domestication technology of Acacia blackwood difficult to root clone tissue culture seedlings, and achieves the effects of improving the quality of seedlings, improving the transplant survival rate and rooting rate, and efficiently cultivating

Inactive Publication Date: 2021-06-18
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above problems, in order to solve the problem of transplanting and surviving the hard-to-root clone tissue culture seedlings of Acacia blackwood, the object of the present invention is to provide a kind of domestication process of the hard-to-root clone tissue-culture seedlings of Acacia blackwood. Carry out special research on the domestication of tissue culture seedlings, so that the survival rate and rooting rate can reach 100%, which greatly improves the quality of seedlings, which is of great significance to improving production efficiency, and can also provide reference for transplanting other difficult-to-root tissue culture seedlings

Method used

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  • The domestication process of tissue culture seedlings of a hard-to-root clone of Acacia blackwood
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  • The domestication process of tissue culture seedlings of a hard-to-root clone of Acacia blackwood

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Experimental program
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Effect test

Embodiment 1

[0031] A kind of domestication process of the hard-to-root clone tissue culture seedling of Acacia blackwood:

[0032] 1) Cultivate rooting induced sprouts: select Acacia blackwood clone tissue culture seedlings, after 14 / 18 days of media rooting induction, the rooting rate is only 23.6% ( figure 2 ), the culture medium takes 1 / 2MS as the basic culture medium, adds IBA1.0mg, white sugar 25g, agar 5g, pH value adjustment value 5.5 in every liter of basic culture medium, after the culture medium is subpackaged, it is prepared in advance at 1.1kg / cm 2 Sterilization under high pressure and high temperature;

[0033] 2) Planting sprouts: Take out the sprouts after rooting induction from the culture bottle, rinse them with clean water, and plant them in a sponge-supported seedling tray. When planting, soak the sponge block, fill the bottom layer with 300ml of pure water, and cover it;

[0034] 3) Cultivation in the culture room: place it in the culture room for 15 days after plant...

Embodiment 2

[0040] A kind of domestication process of the hard-to-root clone tissue culture seedling of Acacia blackwood:

[0041] 1) Cultivate rooting induced sprouts: select Acacia blackwood clone tissue culture seedlings, through 21 days medium rooting induction, the rooting rate is only 36.2% ( Figure 4 ), the culture medium takes 1 / 2MS as the basic culture medium, adds IBA1.5mg, white sugar 30g, agar 6g, pH value adjustment value 6.0 in every liter of basic culture medium, after the culture medium is subpackaged, it is prepared in advance at 1.2kg / cm 2 Sterilization under high pressure and high temperature;

[0042] 2) Planting sprouts: Take out the sprouts after rooting induction from the culture bottle, rinse them with clean water, and plant them in a sponge-supported seedling tray. When planting, soak the sponge block, fill the bottom layer with 300ml of pure water, and cover it;

[0043]3) Cultivation in the culture room: place in the culture room for 20 days after planting, th...

Embodiment 3

[0049] A kind of domestication process of the hard-to-root clone tissue culture seedling of Acacia blackwood:

[0050] 1) Cultivate rooting induced sprouts: select Acacia blackwood clone tissue culture seedlings, through 18 days medium rooting induction, the rooting rate is only 29.3%, the medium is basic medium with 1 / 2MS, in every liter of basic medium Add 1.3mg of IBA, 22g of white sugar, 5.5g of agar, and adjust the pH value to 5.5. 2 Sterilization under high pressure and high temperature;

[0051] 2) Planting sprouts: Take out the sprouts after rooting induction from the culture bottle, rinse them with clean water, and plant them in a sponge-supported seedling tray. When planting, soak the sponge block, fill the bottom layer with 300ml of pure water, and cover it;

[0052] 3) Cultivation in the culture room: place in the culture room for 20 days after planting, the temperature of the culture room is 26°C, and 1700Lx artificial light is added during the day for 11 hours. ...

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Abstract

The invention belongs to the technical field of plant domestication and transplantation, and in particular relates to a domestication process of tissue-cultured seedlings of the hard-to-root clone of Acacia blackwood. The method includes the following steps: 1) Cultivate rooting-inducing sprouts: select the tissue-cultured seedlings of the hard-to-root clone of Acacia blackwood, use medium to induce rooting, and obtain rooting-inducing sprouts; 2) Planting sprouts: plant the sprouts on a sponge support In the seedling tray, soak the sponge block when planting, fill the bottom layer with pure water, and cover it with a cover; 3) Cultivation in the cultivation room: place the seedling tray in the cultivation room, and open the cover every morning and evening for ventilation in the middle stage of cultivation for 10 days. ‑20 minutes; 4) Sunlight room temperature cultivation: transfer the seedling tray to sunlight room temperature, open the cover of the seedling tray throughout the day, and spray once in the morning and evening. It can greatly improve the transplant survival rate and rooting rate of difficult-to-root clone tissue culture seedlings, which can reach 100%, improve the quality of seedlings, shorten the time of nursery cultivation, save nursery management costs, and improve the production efficiency of Acacia black tree tissue culture seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant domestication and transplantation, and in particular relates to a domestication process of tissue-cultured seedlings of the hard-to-root clone of Acacia blackwood. Background technique [0002] Acacia melanoxylon is a tree species of Acacia in Mimosaceae, one of the tallest trees in Acacia, native to Australia, Papua New Guinea and Indonesia. Acacia black wood has beautiful wood grain, good material, nitrogen-fixing root nodules, rich litter, and good soil improvement performance. After being introduced to my country in the 1990s, it was successfully planted in Guangdong, Fujian, Guangxi, Hainan and other places. [0003] Over the past century, our unit has overcome the problems of many branches and indistinct trunks through clonal breeding. Acacia blackwood has become a fast-growing, high-yield, precious timber and ecological public welfare tree species in South China. According to incomplete stat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 裘珍飞范春节曾炳山叶森辉冯家勇
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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