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Stem cell sponge scaffold, preparation method and application

A stem cell and sponge technology, applied in the field of biomaterials and stem cells, can solve the problems of poor adhesion and mechanical properties of gelatin, inflammatory response, and lack of antibacterial ability.

Active Publication Date: 2021-12-07
KYBIOSTEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sponge scaffolds on the market include inorganic salts, gelatin, oxidized cellulose, fibrous collagen, etc., but there are deficiencies, such as inorganic salt sponge scaffolds release heat after absorbing water, and cannot be degraded in vivo; gelatin adhesive properties Poor mechanical properties; oxidized cellulose may cause inflammation; fibrous collagen may cause adverse symptoms such as allergies, etc.
Commonly used polymers for wound healing include polyethylene oxide, polyurethane, and polylactic acid, etc. Such dressings can maintain moisture in the wound and protect tissues from mechanical damage, but cannot activate cell responses, secrete cytokines, and promote wound healing. Heals, also does not have antibacterial ability
At present, there are still relatively few studies on electrospun nanomaterials that can quickly stop bleeding and effectively promote wound healing

Method used

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  • Stem cell sponge scaffold, preparation method and application
  • Stem cell sponge scaffold, preparation method and application
  • Stem cell sponge scaffold, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] This embodiment provides a stem cell sponge scaffold prepared by the following steps:

[0090] (a) Add 0.2 g of chitosan with a viscosity of 200 mPa.s into 10 mL of 85% w / w acetic acid to dissolve, and dissolve 0.7 g of polylactic acid with a weight average molecular weight of 350,000 in 10 mL of hexafluoroisopropanol and stir to dissolve.

[0091] (b) Chitosan solution and polylactic acid solution are mixed evenly according to the weight ratio of 4:6 and then injected into the syringe. At 18KV, a planar nanofibrous membrane was obtained after electrospinning for one hour.

[0092] (c) putting the obtained nanofibrous membrane with planar structure into glutaraldehyde vapor for cross-linking, and cross-linking for 10 hours. Put the cross-linked nanofibers into a vacuum drying oven for drying, and take them out after 3 days.

[0093] (d) Put the dried nanofiber membrane into 0.1mol / L sodium borohydride aqueous solution for foaming, take it out after 10 hours, rinse it ...

Embodiment 2

[0096] This embodiment provides a stem cell sponge scaffold prepared by the following steps:

[0097] (a) 0.4 g of chitosan with a viscosity of 200 mPa.s was dissolved in 10 mL of 85% w / w acetic acid, and 0.7 g of polylactic acid with a weight average molecular weight of 350,000 was dissolved in 10 mL of hexafluoroisopropanol and stirred to dissolve.

[0098] (b) Chitosan solution and polylactic acid solution are mixed evenly according to the weight ratio of 3:7 and then injected into the syringe. The advancing speed of the syringe is adjusted to 1.5mL / h, the distance between the needle and the receiving device is 15cm, and the voltage between the two At 27KV, a nanofibrous membrane with a planar structure was obtained after electrospinning for one hour.

[0099] (c) putting the obtained nanofibrous membrane with planar structure into glutaraldehyde vapor for cross-linking, and cross-linking for 10 hours. Put the cross-linked nanofibers into a vacuum drying oven for drying, a...

Embodiment 3

[0103] This embodiment provides a stem cell sponge scaffold prepared by the following steps:

[0104] (a) Add 0.2 g of chitosan with a viscosity of 400 mPa.s to 10 mL of 85% w / w acetic acid to dissolve, and dissolve 0.7 g of polylactic acid with a weight average molecular weight of 350,000 in 10 mL of hexafluoroisopropanol and stir to dissolve.

[0105] (b) Chitosan solution and polylactic acid solution are mixed evenly according to the weight ratio of 3:7 and then injected into the syringe. The advancing speed of the syringe is adjusted to 1.5mL / h, the distance between the needle and the receiving device is 15cm, and the voltage between the two At 18KV, a planar nanofibrous membrane was obtained after electrospinning for one hour.

[0106] (c) putting the obtained nanofibrous membrane with planar structure into glutaraldehyde vapor for cross-linking, and cross-linking for 10 hours. Put the cross-linked nanofibers into a vacuum drying oven for drying, and take them out after ...

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Abstract

The present invention provides a stem cell sponge scaffold and its preparation method and application, relating to the technical field of biomaterials. The stem cell sponge scaffold provided by the present invention includes a nanofiber sponge and stem cells loaded on the nanofiber sponge. The stem cell sponge scaffold has Good compression and rebound performance can absorb a large amount of wound exudate, keep the wound moist, play a role in protection, hemostasis, and promote wound healing. Moreover, the three-dimensional fiber structure has good mesh connectivity, larger internal surface area and pore size, which can increase the loading quantity of stem cells. In addition, the stem cell sponge scaffold provided by the present invention has good biological activity, biodegradability and biocompatibility, and does not cause inflammation or allergic reaction.

Description

technical field [0001] The invention relates to the technical field of biological materials and stem cells, in particular to a stem cell sponge scaffold and its preparation method and application. Background technique [0002] In accidental injuries caused by wars, traffic accidents and other emergencies, uncontrollable hemorrhage is an important cause of death, and timely and effective hemostasis is very important in trauma first aid and surgical operations. [0003] Traditional sponge supports include gauze, tourniquets, bandages, etc., which can only be used for short-term first aid, and are almost ineffective for bleeding from large blood vessels or large-area wounds. At present, the sponge scaffolds on the market include inorganic salts, gelatin, oxidized cellulose, fibrous collagen, etc., but there are deficiencies, such as inorganic salt sponge scaffolds release heat after absorbing water, and cannot be degraded in vivo; gelatin adhesive properties Poor mechanical pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/00A61L24/04A61L24/08A61L24/10A61L27/38A61L27/18A61L27/20A61L27/22A61L27/24A61L27/54A61L27/56A61L27/58
CPCA61L24/0005A61L24/0015A61L24/0036A61L24/0042A61L24/046A61L24/08A61L24/102A61L24/108A61L27/18A61L27/20A61L27/227A61L27/24A61L27/3834A61L27/54A61L27/56A61L27/58A61L2300/414A61L2400/12
Inventor 温永强张可欣杜宏武
Owner KYBIOSTEM CO LTD
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