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Melting curve abnormal value processing method and device and electronic equipment

A melting curve and processing method technology, applied in the direction of instrumentation, biostatistics, data visualization, etc., can solve the problem that the melting curve cannot be obtained

Pending Publication Date: 2021-04-13
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a melting curve abnormal value processing method to alleviate the technical problem of not being able to obtain a higher-precision melting curve

Method used

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  • Melting curve abnormal value processing method and device and electronic equipment
  • Melting curve abnormal value processing method and device and electronic equipment
  • Melting curve abnormal value processing method and device and electronic equipment

Examples

Experimental program
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Effect test

Embodiment 1

[0054] figure 1 It is a schematic flowchart of a method for processing outliers in melting curves provided in the embodiment of the present application. like figure 1 As shown, the method includes:

[0055] Step S110, acquiring the amplified fluorescence intensity data during the PCR melting process.

[0056] Step S120 , using continuous wavelet transform to detect the fluorescence intensity data, and determine the position of the outlier.

[0057] Step S130, within the preset neighborhood where the outlier position is located, perform linear fitting on the fluorescence intensity data according to the variable window value to obtain a fitting curve.

[0058] Step S140, performing polynomial fitting and derivation on the fitting curve to determine the melting curve.

[0059] By using the continuous wavelet transform to detect the fluorescence intensity data, the outlier position can be determined. Furthermore, the fluorescence intensity data is linearly fitted according to ...

Embodiment 2

[0102] Image 6 A schematic structural diagram of a melting curve outlier processing device provided in the embodiment of the present application, as shown in Image 6As shown, the amplification curve baseline determination device 600 includes:

[0103] An acquisition module 601, configured to acquire amplified fluorescence intensity data during the PCR melting process;

[0104] A detection module 602, configured to detect the fluorescence intensity data by means of continuous wavelet transform, and determine the position of the outlier;

[0105] A fitting module 603, configured to linearly fit the fluorescence intensity data according to a variable window value within the preset neighborhood where the outlier position is located, to obtain a fitting curve;

[0106] The determining module 604 is configured to perform polynomial fitting and derivation on the fitting curve to determine the melting curve.

Embodiment 3

[0108] An electronic device provided by an embodiment of the present application, such as Figure 7 As shown, the electronic device 700 includes a memory 701 and a processor 702, the memory stores a computer program that can run on the processor, and when the processor executes the computer program, the method provided by the above-mentioned embodiments is implemented. step.

[0109] see Figure 7 , the electronic device further includes: a bus 703 and a communication interface 704, the processor 702, the communication interface 704 and the memory 701 are connected through the bus 703; the processor 702 is used to execute executable modules stored in the memory 701, such as computer programs.

[0110] Wherein, the memory 701 may include a high-speed random access memory (Random Access Memory, RAM for short), and may also include a non-volatile memory (non-volatile memory), such as at least one disk memory. The communication connection between the system network element and a...

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Abstract

The invention provides a melting curve abnormal value processing method and device and electronic equipment, and relates to the technical field of data detection, and the method comprises the steps: obtaining amplified fluorescence intensity data in a PCR melting process, carrying out the detection of the fluorescence intensity data in a mode of continuous wavelet transformation, and determining the position of an abnormal value; in the preset neighborhood range where the abnormal value position is located, carrying out linear fitting on the fluorescence intensity data according to the variable window value, obtaining a fitting curve, carrying out polynomial fitting derivation on the fitting curve, determining a melting curve, and solving the technical problem that a high-precision melting curve cannot be obtained.

Description

technical field [0001] The present application relates to the technical field of data detection, in particular to a method, device and electronic equipment for processing abnormal values ​​of melting curves. Background technique [0002] At present, the standard nucleic acid quantification method for diagnosing infectious diseases mostly uses fluorescent real-time quantitative PCR, which can quantify the initial value of the sample template, and is often used in gene analysis and expression, genetically modified food detection and cancer detection. [0003] However, in the current actual data debugging, the fluorescence intensity occasionally has abnormal values, and the existing methods cannot deal with the abnormal values ​​of the melting curve, which is the change value of the fluorescence intensity, and it is difficult to determine the smoothed melting curve. The current method cannot obtain Higher precision melting curve. Contents of the invention [0004] The purpos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B45/00G16B40/00
CPCG16B45/00G16B40/00
Inventor 杨智李冬贺贤汉
Owner HANGZHOU BIOER TECH CO LTD
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