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Tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof

A tetrodotoxin, puffer fish technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problem of losing the ability to produce tetrodotoxin

Active Publication Date: 2020-05-08
ZHEJIANG WANLI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a primary tetrodotoxin-producing tetrodotoxin-producing symbiotic bacterium and its application, which solves the problem that the primary tetrodotoxin-producing puffer symbiotic bacterium Bacillus cereus He-1 is subcultured. Afterwards, the problem of losing tetrodotoxin production capacity

Method used

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  • Tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof
  • Tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof
  • Tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof

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Embodiment 1

[0036] Example 1: The specific steps for recovering tetrodotoxin-producing bacteria's ability to produce tetrodotoxin by using quorum sensing signal molecules are as follows:

[0037] (1) Take the wild puffer fish caught in the Xiangshan sea area of ​​Ningbo, isolate a strain of pufferfish commensal bacteria Bacillus cereus He-1 producing tetrodotoxin from its liver, and store it on the slope; pick the Bacillus cereus stored on the slope The He-1 strain was inoculated in a liquid fermentation medium. The formula of the liquid fermentation medium used was: peptone 5g, yeast extract 5g, beef extract 5g, glucose 4g, sucrose 4g, seawater 1000mL, sterilized at 121°C for 25min, at 180r / min, cultured at 26°C for 18 hours, as seed solution; absorb 20mL seed solution per batch and inoculate into 2L fermentation medium, fermentation medium formula: peptone 4g, yeast extract 4g, beef extract 4g, glucose 3g, sucrose 3g, seawater 1000mL , sterilized at 121°C for 25 minutes, cultured at 18...

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Abstract

The invention discloses a tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof. The tetraodontidae symbiotic bacterium with primary production of tetrodotoxin is named Bacillus cereus He-1, is preserved in the China General Microbiological Culture Collection Center on December 9th, 2019 and has the preservation number of CGMCC NO.19109. The bacillus cereus He-1 is propagated in a culture medium to obtain a bacterial solution of bacillus cereus He-2 which does not produce toxin, and the bacillus cereus is induced to produce tetrodotoxin by adding anautoinducing peptide into the culture solution of bacillus cereus He-2; and the tetrodotoxin producing capacity of the second generation bacterium bacillus cereus He-2 which does not produce toxin isrecovered by adding the autoinducing peptide to serve as a quorum sensing signaling molecule, and tetrodotoxin is produced in a later isolated culture process. The invention solves the problem that atetraodontidae symbiotic bacterium loses the capacity of producing tetrodotoxin after passage of the tetraodontidae symbiotic bacterium with primary production of tetrodotoxin.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a primary tetrodotoxin-producing pufferfish commensal bacterium and an application thereof. Background technique [0002] The consumption of puffer fish has a long history in China, Japan and other Asian countries, and its toxicity has long been known to people. Early studies have established that tetrodotoxin is at the root of the puffer fish's high toxicity. Further studies have also shown that tetrodotoxin is not only present in puffer fish, but widely exists in many species in nature, such as shellfish, molluscs, arthropods, reptiles and amphibians. Tetrodotoxin can not only cause acute food poisoning, but its chronic accumulation in the human body may also be one of the causes of amyotrophic lateral sclerosis (commonly known as "gradual freezing syndrome"). [0003] On the other hand, tetrodotoxin is a neurotoxin with great scientific research an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/18C07D491/22C12R1/085
CPCC12P17/18C12N1/20C07D491/22C12R2001/085C12N1/205
Inventor 袁勇军管峰
Owner ZHEJIANG WANLI UNIV
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