Application of has-miR-320d as molecular marker for radiation exposure diagnosis

A technology of hsa-mir-320d and molecular markers, which is applied in the field of molecular markers for radiation exposure diagnosis, can solve problems that have not been reported, and achieve the effect of quantitative radiation exposure diagnosis

Pending Publication Date: 2020-06-02
CHINA INST FOR RADIATION PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

hsa-miR-320d is related to tumor proliferation, apoptosis, invasion and migration, and has been reported in the diagnosis and prognosis of liver cancer, prostate

Method used

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  • Application of has-miR-320d as molecular marker for radiation exposure diagnosis
  • Application of has-miR-320d as molecular marker for radiation exposure diagnosis
  • Application of has-miR-320d as molecular marker for radiation exposure diagnosis

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Experimental program
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Embodiment 1

[0014] 1. Preliminary screening of miRNA molecular markers for the diagnosis of radiation damage

[0015] Using miRNA high-throughput sequencing technology, by detecting the differential expression of different miRNAs in human peripheral blood 6 hours after 0.2Gy and 2.0Gy γ-ray irradiation, we initially screened miRNA molecular markers for radiation exposure diagnosis.

[0016] 1) Extraction, quantification, quality inspection and purification of total cellular miRNA

[0017] Six hours after human peripheral blood was irradiated with 0.2Gy γ-rays, miRNA was extracted using a peripheral blood miRNA extraction kit, and miRNA samples were subjected to agarose gel electrophoresis and Nanodrop for quality inspection and quantification.

[0018] 2) Construction of library

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Abstract

The invention belongs to the technical field of biological detection and relates to application of has-miR-320d as a molecular marker for radiation exposure diagnosis. Due to application of has-miR-320d as the molecular marker for radiation exposure diagnosis, radiation exposure diagnosis can be implemented conveniently, rapidly, accurately and quantitatively.

Description

technical field [0001] The invention belongs to the technical field of biological detection and relates to the use of hsa-miR-320d as a molecular marker for radiation exposure diagnosis. Background technique [0002] With the development of ionizing radiation technology, the number of workers engaged in radioactive occupations is increasing day by day, and the impact of ionizing radiation on the health of radioactive workers has become a hot issue of high concern. Therefore, how to effectively manage the health of radioactive workers and improve their occupational health has become an important research topic at present. [0003] Occupational health management of radiation workers is one of the key points of radiation health work, including occupational health monitoring. In addition to health status analysis and individual work suitability evaluation, the health monitoring of radiation workers also needs to detect abnormal health changes, prevent the occurrence and develop...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6869C12N15/113
CPCC12Q1/6883C12Q1/6869C12Q2600/158C12Q2600/178C12Q2535/122
Inventor 董娟聪原雅艺王婧洁党旭红孟倩倩张忠新任越王超左雅慧
Owner CHINA INST FOR RADIATION PROTECTION
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