MiR-219a-2-3p and application of miR-219a-2-3p as molecular marker for early diagnosis of radiation damage
A molecular marker, radiation damage technology, applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of quantitative radiation exposure diagnosis
Pending Publication Date: 2022-03-22
CHINA INST FOR RADIATION PROTECTION
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Problems solved by technology
However, there is no report on the relationship between miR-219-2-3p and ionizing radiation exposure at home and abroad
Method used
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Embodiment 1
[0014] 1. Use high-throughput sequencing technology to preliminate radiation-sensitive molecular markers
[0015] 1) Extraction, quantitative and quality inspection of total miRNA
[0016] Human peripheral venous blood was irradiated (at the same time as a non-illuminated control group), 6 h after irradiation, using peripheral blood mRNA extraction kit (Tiangen Biochemical Technology Co., Ltd.) to extract mRNA, mRNA sample is carried out by NanoDrop Quantification (OD260 / 280: 1.81-1.87, Total: 1699.50-3592.80 ng).
[0017] 2) Construction of the library
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The invention belongs to the technical field of biological detection, and relates to miR-219a-2-3p and application thereof as a molecular marker for early diagnosis of radiation damage. According to the application of the miR-219a-2-3p as the molecular marker for early diagnosis of radiation damage, radiation exposure diagnosis can be conveniently, accurately and quantitatively carried out.
Description
Technical field [0001] The present invention belongs to the use of biological detection techniques, and the use of MIR-219A-2-3P and its early diagnosis of radiation injury. Background technique [0002] At present, the health care of radiopters is mainly health checkup, health risk assessment and disease prevention and treatment. Daily health checkups mainly adopt conventional clinical inspections, special health checkups include chromosome distortion and micronuclear analysis. However, the indicators they involve do not effectively avoid the occurrence of the disease caused by radiation. [0003] The molecular indicators obtained by molecular biology related technologies can be supplemented and improved physical examination means, and the disease is early diagnosis, early treatment. Therefore, in order to improve the current health care system, avoid or reduce the potential risks of radiation hazards, and urgently need to establish early health care techniques for radiosalists,...
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IPC IPC(8): C12N15/113C12Q1/6883
CPCC12N15/113C12Q1/6883C12Q2600/178C12Q2600/158C12N2310/141
Inventor 董娟聪原雅艺党旭红杨彪程娇孟倩倩王超张忠新柴栋静左雅慧
Owner CHINA INST FOR RADIATION PROTECTION