MiR-219a-2-3p and application of miR-219a-2-3p as molecular marker for early diagnosis of radiation damage
A molecular marker, radiation damage technology, applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of quantitative radiation exposure diagnosis
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[0014] 1. Use high-throughput sequencing technology to preliminate radiation-sensitive molecular markers
[0015] 1) Extraction, quantitative and quality inspection of total miRNA
[0016] Human peripheral venous blood was irradiated (at the same time as a non-illuminated control group), 6 h after irradiation, using peripheral blood mRNA extraction kit (Tiangen Biochemical Technology Co., Ltd.) to extract mRNA, mRNA sample is carried out by NanoDrop Quantification (OD260 / 280: 1.81-1.87, Total: 1699.50-3592.80 ng).
[0017] 2) Construction of the library
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