Hsa-miR-23c and application thereof as molecular marker for early diagnosis of radiation damage
A technology of hsa-mir-23c and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems that have not been reported, and achieve the effect of quantitative radiation exposure diagnosis
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[0014] 1. Use high-throughput sequencing technology to preliminate radiation-sensitive molecular markers
[0015] 1) Extraction, quantitative and quality inspection of total miRNA
[0016] The peripheral venous blood was irradiated with 0.2 Gy gamma ray (at the same time as a non-irradiated control group), 6 h after irradiation, using peripheral blood miRNA extraction kit (Tiangen Biochemical Technology Co., Ltd.) to extract miRNA, miRNA sample through agarose gel Electrophoresis and NanoDrop perform quality test (OD260 / 280: 1.80-1.89, total: 1699.50-3125.40 ng).
[0017] 2) Construction of the library
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