LincR-0003 and application thereof as molecular marker for early diagnosis of radiation damage
A molecular marker, radiation damage technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems such as unreported
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[0014] 1. Preliminary screening of radiation-sensitive molecular markers using lincRNA chip technology
[0015] 1) Extraction, quantification and quality inspection of total cellular RNA
[0016] Human peripheral venous blood was irradiated with 0.2Gy γ-rays (a non-irradiated control group was set at the same time), and 6 hours after irradiation, RNA was extracted with a peripheral blood RNA extraction kit (Tiangen Biochemical Technology Co., Ltd.), and the RNA samples were quality-tested by Nanodrop Quantitative (OD260 / 280: 1.80-1.88, total amount: 2181.80-3558.80ng).
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