MiR-1287-5p and application of miR-1287-5p as molecular marker for early diagnosis of radiation damage
A technology of molecular markers and radiation damage, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of quantitative radiation exposure diagnosis
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[0014] 1. Preliminary screening of radiation-sensitive molecular markers using high-throughput sequencing technology
[0015] 1) Extraction, quantification and quality inspection of total cellular mRNA
[0016] Human peripheral venous blood was irradiated with 0.2Gy γ-rays (at the same time, a non-irradiated control group was set), and 6 hours after irradiation, mRNA was extracted with a peripheral blood mRNA extraction kit (Tiangen Biochemical Technology Co., Ltd.), and the mRNA samples were quality-tested by Nanodrop Quantitative (OD260 / 280: 1.80-1.89, total amount: 1699.50-3125.40ng).
[0017] 2) Construction of library
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