MiR-1287-5p and application of miR-1287-5p as molecular marker for early diagnosis of radiation damage
A technology of molecular markers and radiation damage, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of quantitative radiation exposure diagnosis
Pending Publication Date: 2022-03-01
CHINA INST FOR RADIATION PROTECTION
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Problems solved by technology
However, there is no report on the relationship between miR-1287-5p and exposure to ionizing radiation at home and abroad.
Method used
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Embodiment 1
[0014] 1. Preliminary screening of radiation-sensitive molecular markers using high-throughput sequencing technology
[0015] 1) Extraction, quantification and quality inspection of total cellular mRNA
[0016] Human peripheral venous blood was irradiated with 0.2Gy γ-rays (at the same time, a non-irradiated control group was set), and 6 hours after irradiation, mRNA was extracted with a peripheral blood mRNA extraction kit (Tiangen Biochemical Technology Co., Ltd.), and the mRNA samples were quality-tested by Nanodrop Quantitative (OD260 / 280: 1.80-1.89, total amount: 1699.50-3125.40ng).
[0017] 2) Construction of library
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The invention belongs to the technical field of biological detection, and relates to miR-1287-5p and application thereof as a molecular marker for early diagnosis of radiation damage. According to the application of the miR-1287-5p as the molecular marker for early diagnosis of radiation damage, radiation exposure diagnosis can be conveniently, accurately and quantitatively carried out.
Description
technical field [0001] The invention belongs to the technical field of biological detection, and relates to miR-1287-5p and its use as a molecular marker for early diagnosis of radiation damage. Background technique [0002] At present, the health monitoring of radiation workers mainly includes physical examination, health risk assessment and disease prevention and treatment. Daily health checkups mainly use routine clinical examinations, and special health checkups include chromosomal aberrations and micronucleus analysis. However, the indicators they involve cannot effectively detect the occurrence of radiation-induced diseases at an early stage. [0003] Molecular indicators obtained by molecular biology-related technologies can supplement and improve physical examination methods to achieve early diagnosis and early treatment of diseases. Therefore, in order to improve the existing health monitoring system and avoid or reduce the potential risks of radiation hazards, it...
Claims
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IPC IPC(8): C12N15/113C12Q1/6883
CPCC12N15/113C12Q1/6883C12N2310/141C12Q2600/158C12Q2600/178
Inventor 董娟聪原雅艺党旭红柴栋良杨彪孟倩倩程娇王超刘红艳左雅慧
Owner CHINA INST FOR RADIATION PROTECTION