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Application of miR-6858 in preventing and treating glioma through melatonin

A technology of mir-6858 and melatonin, applied in the field of biomedicine, can solve problems such as incomplete clarity

Active Publication Date: 2020-06-05
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that whether MEL exerts its anti-GBM effect by regulating miRNA is still not fully understood

Method used

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  • Application of miR-6858 in preventing and treating glioma through melatonin
  • Application of miR-6858 in preventing and treating glioma through melatonin
  • Application of miR-6858 in preventing and treating glioma through melatonin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0106] 1 Materials and methods

[0107] 1.1 Ethics statement

[0108] All animal experiments were approved by the Animal Care and Use Committee of Shandong University (IACUC).

[0109] 1.2 Cell lines and cell culture

[0110] Human glioma cell lines U87, U251, and A172 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai; TCHu138, TCHu58, and TCHu171). GBM#P3 provided by Prof. Rolf Bjerkvig, University of Bergen. DMEM medium (Thermo Fisher Scientific, SH30022.01B), 10% fetal bovine serum (GE Healthcare Life Sciences, 10082147), 5% CO2, placed in a humidified incubator at 37°C.

[0111] 1.3 Cell Viability and Proliferation Assays

[0112] Cell viability was detected by CCK-8 kit (Dojindo, CK04-500). cells (1.0×10 4 cells / well) were seeded in 96-well plates and incubated overnight at 37°C. MEL (Abcam, ab141933) was dissolved in DMSO (Sigma-Aldrich, D2650) and diluted to a working concentration in culture medium. After necessary treatment, the ce...

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Abstract

The invention provides application of miR-6858 in preventing and treating glioma through melatonin and belongs to the technical field of biomedicines. Specifically, the invention finds that melatoninhas a good antitumor function on cells U251, U87, A172 and GBM#P3. Meanwhile, through miRNA (micro ribonucleic acid) sequencing on GBM (glioblastoma multiforme) cells treated by using melatonin, target miRNA is found, growth of the GBM cells can be inhibited by increasing miR-6858 through the melatonin, and furthermore, an SIRT3 / AKT signal channel can be inhibited. The invention illuminates molecule mechanisms based on interactions melatonin and miRNA, and an effective treatment strategy is provided for treatment on GBM.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of miR-6858 in preventing and treating glioma with melatonin. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Glioblastoma multiforme (GBM) is the most common and deadly primary malignancy in the brain. Although some progress has been made in surgery and comprehensive treatment for glioblastoma patients, the life expectancy of diagnosed patients is only 12-14 months. Therefore, new therapeutic strategies are urgently needed to improve the prognosis of glioblastoma patients. [0004] MicroRNAs (miRNAs) are small, highly conserved non-coding RNA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4045A61K45/06A61K31/7088A61P35/00
CPCA61K31/4045A61K31/7088A61K45/06A61P35/00A61K2300/00
Inventor 黄斌李新钢王剑王东海王成龙赵志敏
Owner SHANDONG UNIV QILU HOSPITAL
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