The invention relates to the technical field of organoid culture, and particularly discloses a culture method for screening tumor organoids based on P53 mutation. The culture method comprises the following steps: S1, unfreezing untreated tumor organoid to generate a cell suspension, adding the cell suspension into a basic culture medium, uniformly conducting mixing and centrifuging, and removing supernate to obtain a tumor organoid mixture; S2, adding matrigel to resuspend the tumor organoid mixture, and after resuspending, inoculating the organoid mixture into an organoid culture medium containing y27632 for culturing; and S3, after the liquid of the tumor organoid mixture is changed for the first time, absorbing and removing the organoid culture medium, and then adding a culture medium containing Nutlin3A for continuous culture. The culture method is low in cost and price, sequencing is not needed, the detection cost is saved in the early stage of culture of the tumor organoids, and accurate and simplified culture of the tumor organoids is achieved.