12results about How to "Induced death" patented technology

The invention discloses a copper ion carrier material with a photothermal effect, a preparation method and an anti-cancer drug formed by the copper ion carrier material, and belongs to the technical field of nano drug loading technologies, and the structural formula of the copper ion carrier material is as follows: # imgabs0 #. The preparation method comprises the following steps: S1, mixing butanedione and concentrated hydrochloric acid in water, stirring and reacting, adding 4-methyl thiosemicarbazide, continuously stirring and reacting, and performing post-treatment to obtain a compound (4); then dissolving the compound (3) and 4, 4-dimethyl thiourea in a DMSO solvent, dropwise adding glacial acetic acid for reaction, and performing post-treatment to obtain a compound (6); dissolving the compound (6) and 6-aminocaproic acid in an acetonitrile solvent, reacting, and performing post-treatment to obtain a compound (8); and dissolving the compound (8) and beta-cyclodextrin in a DMSO solvent, mixing, adding an activating agent and a condensing agent, reacting, and performing post-treatment to obtain the copper ion carrier material with the photothermal effect. The problems that a copper ion carrier is poor in water solubility, low in photothermal conversion efficiency and low in near-infrared light energy, ultraviolet light is not prone to penetrating into tissues, and tissue damage can be caused are solved.

On the first aspect, the emodin nano preparation based on the copper ions is provided, emodin is used as a copper ion carrier, and emodin and the copper ions synergistically play a role in inhibiting tumor cell activity; on the second aspect, the invention provides a preparation method of the anti-tumor nano preparation, the particle size of the preparation is controlled to be 100-200nm, the distribution and residence time of the active ingredients in tumor tissues are improved, and the preparation has tumor targeting property. And on the third aspect, the ROS content of the emodin in tumor cells is increased by utilizing the photodynamic effect, and the activity of the tumor cells is inhibited.

The invention provides a nano medicine for promoting tumor cell copper death and a preparation method thereof, and the preparation method comprises the following steps: selecting a nano raw material carrier, combining copper ion protein, loading a copper ion chelating agent, carrying out ultrasonic wall breaking processing, carrying out surface decoration on the nano carrier, dispersing copper ions, and carrying out dry-method whole particle preparation. According to the nano-drug, the copper ions are carried by the specific nano-carrier, so that the copper ions can be selectively released in tumor cells, the tumor cells are induced to generate copper-dependent death, cell metabolism disorder can be caused by imbalance of a copper steady state, synthesis of iron-sulfur cluster proteins related to mitochondrial respiration is inhibited, and the tumor cell death rate is increased. According to the present invention, with the application of the polypeptide as the protein lipoic acylation modification upstream regulatory factor, the polypeptide can be directly combined with and induce the heteropolymerization, and the drug has good biocompatibility and good targeting property, and the polypeptide can be used as the protein lipoic acylation modification upstream regulatory factor.

The present invention belongs to the field of tumor immunotherapy, and specifically relates to a nano-immunomodulator F@PDA@I and its application in tumor immunotherapy, wherein the nano-immunomodulator integrates the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione oxidase (GSHOx). Photodynamic therapy (PDT) and photothermal therapy (PTT) are used to achieve tumor immunotherapy. The multi-enzyme-like activity not only contributes to the generation of oxygen to relieve hypoxia and promotes the polarization of M2 to M1 macrophages, but also consumes GSH in the TME to promote PDT and induce ferroptosis. As a result, a large amount of necrotic debris is released, which ultimately promotes the maturation of dendritic cells and the infiltration of cytotoxic T lymphocytes, thereby reversing the immunosuppressive TME. In in vivo experiments, F@PDA@I is combined with a Toll-like receptor agonist for synergistic immunotherapy against primary and distal tumors.

The invention relates to the technical field of nano biological medicines, and discloses a photo-thermal-enzyme catalysis nano-particle as well as a preparation method and application thereof. The photo-thermal-enzyme catalysis nano-particles are constructed by crosslinking water-soluble self-doped polyaniline nano-particles, human serum albumin, lactate oxidase and collagenase through a thioacetal linker capable of being split by active oxygen. Collagenase and lactate oxidase (LOx) are integrated into the same nanoparticle system through a thioacetal (TK) linker, the TK linker is split under the action of tumor microenvironment active oxygen (ROS) and H2O2 generated by catalysis of the LOx, the activity of the two enzymes is synchronously activated, a collagen matrix (physical barrier) is degraded, lactic acid (chemical barrier) is removed, the tumor microenvironment is efficiently remodeled, and the tumor microenvironment can be effectively inhibited. The problems that the regulation effect of a single barrier is limited, and the tumor microenvironment is difficult to improve are solved.

The present application relates to the technical field of biological products, and provides an application of Ganoderma lucidum exosomes in the treatment of brain glioma, an extraction method and a composition, and the application of Ganoderma lucidum exosomes in any one or more of the following: a) preparing a product that promotes apoptosis of brain glioma cells; b) preparing a product that inhibits proliferation of brain glioma cells; c) preparing a product that inhibits autophagy of brain glioma cells; d) preparing a product for treating brain glioma. The present application verifies through cell and animal experiments that Ganoderma lucidum exosomes can effectively induce apoptosis of brain glioma cells and inhibit autophagy of brain glioma cells to induce glioma death, and act on brain glioma cells through the AKT / mTOR signaling pathway and inhibiting the production of autophagosomes in brain glioma cells.

The present invention relates to a composition for preventing or treating cancer diseases, the composition comprising CD8+T cells activated by extracellular vesicles derived from CD4+T cells as an active ingredient. It was found that the extracellular vesicles secreted from cytokine-activated CD4+T cells increased, and the extracellular vesicles enhanced the proliferation and activity of CD8+T cells to induce cancer cell death, thereby enhancing the anti-cancer effect. Therefore, the present invention provides CD8+T cells activated by extracellular vesicles derived from CD4+T cells as a medicament or immunotherapeutic agent for cancer diseases, and a method for activating CD8+T cells using extracellular vesicles derived from CD4+T cells to prepare CD8+T cells exhibiting excellent anti-cancer activity as described above.

This invention relates to the field of biomedical technology, specifically to the application of an organic photosensitive acid generator in the preparation of antitumor drugs. In this invention, an organic photoresponsive molecular system is constructed. The organic photosensitive acid generator possesses the synergistic effect of photoacid therapy (PAT) and photodynamic therapy (PDT), enhancing the efficacy of photoactivated therapy and improving the reliability and effectiveness of tumor treatment. Under ultraviolet light irradiation of a specific wavelength, the organic photosensitive acid generator produces a large amount of H₂. + Reactive oxygen species (ROS) can damage the DNA, proteins, and lipids of tumor cells, thereby inducing cell death.

The invention discloses a medicine of a combination of Complete and an iron agent and a preparation method and application thereof, and belongs to the technical field of medicines.The medicine of the combination of Complete and the iron agent comprises the composition of Complete and the iron agent, the iron agent is a Fe < 3 + > donor, and the Fe < 3 + > donor can be selected from ferric salt or iron oxide of ferric iron or nano-particles of ferric salt or iron oxide. The pharmaceutical composition also comprises a pharmaceutically acceptable carrier and an additive, the inhibition effect of the pharmaceutical composition on the growth of tumor cells is obviously superior to the effect of independently using the Complete, the condition of drug resistance of the tumor cells to the Complete is improved, the pharmaceutical composition can be applied to preparation of the medicine for treating squamous cell carcinoma of lung, and the use dosage of the Complete can be reduced.

The present invention discloses a functional drug-carrying small molecule peptide that promotes ferroptosis of breast cancer cells, and belongs to the technical field of the establishment and preparation of small molecule peptide nano drug carrier systems. The functional small molecule peptide responds to the slight acidity in the tumor microenvironment and can be used as a drug carrier, and the structural formula is shown in Figure 1. The functional drug-carrying small molecule peptide system PS / Pep1 of the present invention can target the coagulation plasma protein in tumor blood vessels, and can increase the cytotoxicity to tumor cells and reduce the toxic side effects to normal cells. PS / Pep1 undergoes a morphological change in the tumor microenvironment, and it co-loads PTX and SF, which increases the retention time of the combined drug in tumor cells. Compared with PS / Pep2, which cannot be morphologically transformed, PS / Pep1 more effectively inhibits tumor growth. More importantly, PS / Pep1 can effectively induce ferroptosis of breast cancer cells.

This invention discloses a ZIF-8 nanomedicine delivery system co-loaded with doxorubicin and siTDRKH, its preparation method, and its applications, belonging to the fields of biomedicine and nanomaterials technology. The nanomedicine delivery system uses the zeolite imidazole ester framework material ZIF-8 as a carrier. Its porous structure encapsulates the chemotherapeutic drug doxorubicin within the framework, and small interfering RNA targeting the TDRKH gene is loaded onto the surface through electrostatic adsorption. This structure exhibits high pH responsiveness: it remains stable under normal physiological conditions (pH 7.4), preventing premature drug leakage; upon entering the slightly acidic environment of a tumor (pH 6.5) or a lysosomal environment (pH 5.0), the ZIF-8 framework immediately disintegrates, achieving targeted and synergistic release of the loaded drug.

The present invention belongs to the technical field of biomedical materials, and specifically relates to an anti-tumor nano drug delivery system containing β-ketoenamine and a preparation method and application thereof. The anti-tumor nano drug delivery system is based on COF TFP‑DABP As a carrier, after loading the drug, RVG-29 modified with double-terminal amino-PEG was grafted on the surface to obtain OT@COF-RVG. The anti-tumor nano drug delivery system of the present invention uses a type I photosensitive covalent organic framework material containing a β-ketoenamine configuration as a carrier platform, integrates TMZ and OSMI-4 into the COF cavity, and then modifies the surface with polyethylene glycol and rabies virus peptide RVG-29, which can effectively activate non-oxygen-consuming photodynamic therapy to promote cell death, inhibit O-GlcNAc to promote lysosomal degradation of PD-L1, and enhance the effectiveness of immune checkpoint blockade therapy, thereby significantly improving its anti-tumor effect.