Intestinal microbial marker for distinguishing autoimmune hepatitis patients from healthy people and applications of intestinal microbial marker

An autoimmune and gut microbiome technology, applied in the field of biomedicine, can solve the problems that have not been reported in gut microbiome models, and achieve the effect of good discrimination ability.

Pending Publication Date: 2020-06-09
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, gut microbial models for distinguishing autoimmune hepatitis from healthy people have not been reported

Method used

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  • Intestinal microbial marker for distinguishing autoimmune hepatitis patients from healthy people and applications of intestinal microbial marker
  • Intestinal microbial marker for distinguishing autoimmune hepatitis patients from healthy people and applications of intestinal microbial marker
  • Intestinal microbial marker for distinguishing autoimmune hepatitis patients from healthy people and applications of intestinal microbial marker

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Embodiment Construction

[0050] The present invention will be further described below in conjunction with the examples, but the protection content of the present invention is not limited to these examples.

[0051] The methods used in the following examples are conventional methods unless otherwise specified. The materials or reagents required in the following examples were obtained from open commercial channels unless otherwise specified.

[0052] The present invention collects the feces samples of the enrolled subjects, extracts the total DNA of microorganisms, and performs 16SrDNA Miseq sequencing of the intestinal flora. Based on the high-throughput sequencing data, a microbial discrimination model between autoimmune hepatitis and healthy people was established in the training set, and the prevalence of autoimmune hepatitis (probability of disease, POD) index was established; the POD index can be used to calculate its discrimination ability, and the verify.

[0053] The operation steps are as fo...

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Abstract

The invention belongs to the technical field of biomedicine, and specifically relates to an intestinal microbial marker for distinguishing autoimmune hepatitis patients from healthy people and applications of the intestinal microbial marker. The intestinal microbial marker for distinguishing the autoimmune hepatitis patients from the healthy people provided by the invention is composed of microorganisms indicated by at least one gene shown in SEQ ID NO:1-5, and the microbial genes are obviously enriched or reduced in human intestines. The invention also provides an application of a detection reagent to preparation of a detection kit for distinguishing the autoimmune hepatitis patients from the healthy people, and an application of the detection reagent to establishing of an intestinal microbial model for distinguishing the autoimmune hepatitis patients from the healthy people. Therefore, the microbial gene distinguishing model provided by the invention realizes good distinguishing ability in the autoimmune hepatitis patients and the healthy people.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an intestinal microbial marker for distinguishing autoimmune hepatitis from healthy people and its application. Background technique [0002] Autoimmune hepatitis (AIH) is a chronic, nonspecific liver inflammation mediated by the immune system. Epidemiological statistics show that the incidence rate of autoimmune liver in China is as high as 20 / 100,000, and the annual average incidence rate of Nordic whites is 1.07-1.9 / 100,000. Its etiology is related to multiple genetic and environmental factors, but has not been fully elucidated. Long-term untreated autoimmune hepatitis will increase the risk of cirrhosis and / or hepatocellular carcinoma (HCC), and the mortality rate of multiple complications such as intractable ascites and upper gastrointestinal bleeding is common in the decompensated stage of cirrhosis It is very high and poses a serious threat to the health o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6869C12N15/11G16B40/20G16H50/20G16H50/30
CPCC12Q1/689C12Q1/6869G16B40/20G16H50/30G16H50/20C12Q2535/122
Inventor 任志刚楼佳敏余祖江
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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