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Immunologic mechanism and treatment drug for gastrointestinal diseases

A technology for gastrointestinal diseases and drugs, applied in the field of gastrointestinal diseases, can solve the problems of lack of specific treatment methods for functional gastrointestinal diseases, improve the level of clinical personalized diagnosis and treatment, and promote accurate classification type effect

Active Publication Date: 2020-06-30
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Treatment of functional gastrointestinal disorders in children lacks specific therapeutic approaches due to lack of understanding of etiology and pathogenesis

Method used

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  • Immunologic mechanism and treatment drug for gastrointestinal diseases
  • Immunologic mechanism and treatment drug for gastrointestinal diseases
  • Immunologic mechanism and treatment drug for gastrointestinal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1. Single-cell sequencing and bioinformatic analysis of cells in the colonic mucosa of children with colitis and controls

[0102] I. Research program:

[0103] (1) Obtain colonic mucosal tissue from children with control and non-infectious gastrointestinal diseases, and obtain single cell suspension;

[0104] (2) After surface staining, four subpopulations of T, B, non-TB lymphocytes, and non-lymphocytes were sorted by flow cytometry;

[0105] (3) Single-cell transcriptome analysis for each cell subpopulation;

[0106] (4) Single-cell sequencing results, analyzing the clustering characteristics of immune cell subsets, and establishing the composition and transcriptional characteristics of colonic mucosal immune cells;

[0107] (5) Determine the specific functional characteristics of cell subgroups through signal pathway enrichment analysis. Identify cell-type-specific highly expressed transcription factors, and establish transcription factor regulatory netwo...

Embodiment 2

[0131] Example 2. Clinical trials and evaluation of dipyridamole in the treatment of colitis in children

[0132] The following study was carried out on the colonic mucosa of the patients described in Example 1, ie children with non-eosinophilic colitis and eosinophilic colitis (67 and 23 cases, respectively), and 28 control children. Please refer to Table 3 for the condition of the patients.

[0133] Table 3. Clinical characteristics of control and disease groups

[0134]

[0135]

[0136]

[0137] *P<0.01 and **P<0.001 are compared with the control group; #P<0.01 and ##P<0.001 are compared with the non-eosinophilic colitis group.

[0138] It was investigated whether reduced expression of CD39 in the colonic mucosa exacerbates inflammation by promoting ATP and ADP accumulation. First, we correlated CD39 expression on T cells with ATP and ADP excretion in colon biopsies stored overnight. In these cases, ATP cannot be detected. However, the decreased expression of ...

Embodiment 3

[0156] The effect of dipyridamole in DSS (dextran sulfate sodium, dextran sulphate sodium)-induced acute colitis model

[0157] mouse

[0158] Six- to eight-week-old male C57BL / 6J mice (20-22 g) were purchased from Guangzhou University of Traditional Chinese Medicine. The mice were given regular food and water before the experiment. Weight-matched mice were used in all studies. Animal studies were approved by the Animal Care and Use Committee of Guangzhou Medical University (permit number: 2019-471) and performed according to institutional guidelines.

[0159] DSS-induced acute colitis

[0160]Mice were pretreated with dipyridamole (10 mg / kg body weight, Sigma-Aldrich) or vehicle (2% DMSO, 10% ethanol, 88% corn oil) twice daily for 3 days, followed by DSS (36,000– 50,000MW), MP Biomedicals) were added to their drinking water (3%) for 9 days. Control mice were given regular food and water. Mice were monitored daily and euthanized if they lost more than 20% of their body w...

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Abstract

The present invention relates to an immunologic mechanism for gastrointestinal diseases such as non-infectious gastrointestinal diseases including child non-infectious gastrointestinal diseases, and anovel application of a phosphodiesterase inhibitor and / or an antiplatelet drug to treatment of the gastrointestinal diseases such as the non-infectious gastrointestinal diseases.

Description

technical field [0001] The present invention relates to gastrointestinal diseases, such as infectious or non-infectious gastrointestinal diseases, in particular non-infectious gastrointestinal diseases, including non-infectious gastrointestinal diseases in children and the new application of PDE inhibitors and / or antiplatelet drugs in the treatment of gastrointestinal diseases, such as non-infectious gastrointestinal diseases. Background technique [0002] Gastrointestinal diseases, such as non-infectious gastrointestinal diseases, are a general term for a variety of gastrointestinal inflammatory diseases, including, for example, inflammatory gastrointestinal diseases (such as inflammatory bowel disease (inflammatory bowel disease, hereinafter Sometimes referred to simply as IBD), allergic gastrointestinal disorders (such as food allergies), and functional gastrointestinal disorders can affect people of all ages. It is also especially common in infants and young children, a...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K45/06A61K31/519A61P1/00
CPCA61K45/00A61K45/06A61K31/519A61P1/00A61K31/00A61K31/573A61P1/06G01N2800/065G01N2800/222G01N2333/70596G01N2333/525G01N33/56972G01N33/86G01N33/5041
Inventor 张玉霞杨敏黄冰陈章华张莉白帆龚四堂
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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