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A method for the separation, purification and large-scale cultivation of Daphnia spindle

A technology of separation, purification and culture method, which is applied in the field of Daphnia fusiformis separation, purification and large-scale culture, and can solve the problem of high inability

Active Publication Date: 2021-10-22
ZHEJIANG OCEAN UNIV
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the problem that the existing water flea breeding methods cannot achieve high-purity cultivation of a single species, and provide a method for separation, purification and large-scale cultivation of water fleas, which can greatly improve the breeding purity of water fleas

Method used

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  • A method for the separation, purification and large-scale cultivation of Daphnia spindle
  • A method for the separation, purification and large-scale cultivation of Daphnia spindle

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Embodiment

[0021] Such as figure 1 , figure 2 In the shown embodiment, a method for separation, purification and large-scale cultivation of Daphnia spindle is carried out according to the following steps:

[0022] Step 1. Acquisition of copepods: In the offshore area, when the water temperature is 20±1°C, at the transition from spring to summer, use a plankton trawl net made of 100-mesh silk screen to harvest copepods at the surface of the 20-60cm water body population as a source of germplasm for screening;

[0023] Step 2. Directional screening of copepods: place the copepods at a temperature of 20±1°C, a salinity of 30°C, and a light intensity of 50-200 Lux and cultivate them for a week, then raise the water temperature to 25±1°C, and the salinity drops. To 20, after a week of cultivation, return to the initial conditions: 20±1°C temperature, 30 salinity, light intensity 50-200 Lux for a week, that is, complete a cycle of directional screening, after completing a cycle of screening...

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Abstract

The invention discloses a method for the separation, purification and large-scale cultivation of daphnia spindle, which is carried out according to the following steps: step 1, obtain copepods; step 2, directional screening of copepods; step 3, expand cultivation. The invention improves the culturing purity of the spinach daphnia, and the cultivating device has a simple structure and is suitable for large-scale culturing.

Description

technical field [0001] The invention relates to a method for cultivating water fleas, in particular to a method for isolating, purifying, and cultivating water fleas on a large scale. Background technique [0002] Copepods are widely distributed in oceans, freshwater or brackish water, and are an important part of zooplankton in waters, as well as an important link in food webs in waters. Because its different species are distributed in different seasons and have high nutritional value, it is the opening bait for the larvae of many economic animals when raising seedlings. Therefore, it is necessary to carry out the research on nutritional enhancement and large-scale cultivation of copepods. Carrying out research on nutrition enhancement and large-scale breeding of copepods can not only provide basic bait for seedling bait research, but also provide scientific basis for the construction and utilization of fish farms based on large-scale breeding of copepods; for example, a k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/20A01K61/90
CPCA01K61/20A01K61/90
Inventor 周超金扬湖张建设
Owner ZHEJIANG OCEAN UNIV
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