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Application of combination of low-temperature plasma and metformin

A technology of low-temperature plasma and metformin, applied in parts of surgical instruments, medical preparations containing active ingredients, medical science, etc., can solve problems such as unclear effects and achieve the effect of inducing cell death

Active Publication Date: 2020-07-31
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although TMZ can provide positive therapeutic effects in patients with high-grade glioma, its role in low-grade glioma and some other glioma subtypes with genetic mutations is not well understood

Method used

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  • Application of combination of low-temperature plasma and metformin
  • Application of combination of low-temperature plasma and metformin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Determination of the inhibitory effect of low temperature plasma (CAP) and metformin (MET) alone or in combination on U251 / U87 glioma cells

[0022] 1. In order to determine whether the independent action of low-temperature plasma and metformin has an effect on the cell viability of the glioma cell line, in this embodiment, the effects of low-temperature plasma and metformin on the viability of the glioma cell line were first detected respectively. The U251 cell line was divided into 7.5*10 4 cells / ml, U87 cell line 5*10 4 Inoculated in 96-well plate at a density of / ml, after 24 hours of culture:

[0023] (1) Plasma alone effect experiment: according to the low-temperature plasma treatment time of 10, 20, 40, and 60 seconds, respectively, after 24 hours and 48 hours of treatment, the cell viability was detected by the CellTiter Blue method. The results are as follows figure 1 As shown in A-1B, it can be seen from the results in the figure that low-temperat...

Embodiment 2

[0029] Example 2: Determination of the effect of the combination of low temperature plasma and metformin inducing the death of U251 / U87 glioma cells

[0030] After culturing U251 and U87 glioma cells in a 96-well plate for 12 hours, add 16 mmol / L of metformin, continue culturing for 12 hours, and then treat them with low-temperature plasma for 30 seconds, and continue culturing for 12 hours. The results are as follows: figure 2 As shown in A-2B; it can be seen from the figure that the combination of low-temperature plasma and metformin can change the shape of the cells, making the cells round and showing a death-like shape. As detected by the cell viability kit, compared with a single treatment, the combined use can indeed significantly induce cell death (such as figure 2 C-2D, living cells are in the circle, and dead cells are in the square; figure 2 E-2F is the statistical graph of cell death rate).

[0031] figure 2 In A-2B, the glioma cell line U251 ( figure 2 A),...

Embodiment 3

[0034] Example 3 Carry out further analysis experiments on the synergistic inhibitory effect mechanism of low temperature plasma and metformin

[0035] U251 and U87 glioma cells were cultured in a 96-well plate for 24 hours, then treated with low-temperature plasma for 30 seconds, and the level of free oxygen in cells was detected with a reactive oxygen species detection kit and detected with a hydrogen peroxide kit. Hydrogen peroxide levels in low-temperature plasma-treated cell culture media.

[0036] like image 3 A, glioma cell lines U251 (above) and U87 cell lines (below) were cultured for 12 hours, treated with CAP for 30 seconds, and the level of free oxygen in cells was detected with a reactive oxygen species detection kit (inside the circle is intracellular active oxygen), and found that CAP treatment significantly increased the level of free oxygen in cells.

[0037] like image 3 B. The hydrogen peroxide level in the CAP-treated cell culture medium was detected w...

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Abstract

The invention discloses application of combination of a low-temperature plasma and metformin, in particular to application of the combination of the low-temperature plasma and metformin to in-vitro non-therapeutic induction of glioma cell death. The application of the combination of the low-temperature plasma and the metformin to in-vitro non-therapeutic induction of glioma cell death shows that the combination of the low-temperature plasma and the metformin has an obvious synergistic inhibition effect on glioma cell lines U251 and U87 cultured in vitro, and can remarkably induce cell death. The application is used for non-disease treatment, but for in vitro studies, through the scheme of the application of the combination of the metformin and the low-temperature plasma to inducing gliomadeath, a new strategy can be provided for researching the application of the combination of the low-temperature plasma and the metformin to glioma treatment, and a new thought is expected to be provided for designing a new product or a new scheme for treating glioma.

Description

technical field [0001] The invention relates to the new application of low-temperature plasma and metformin in the field, in particular to an application of low-temperature plasma combined with metformin. Background technique [0002] Glioma is the most common primary brain tumor, among which malignant glioma (GBM) is the most malignant glioma, and its five-year survival rate is about 5%. Tumor survival has not been effectively improved. The incidence of glioma in China is about 4 / 100,000. According to the classification of central nervous system tumors in the World Health Organization in 2016, gliomas are divided into peripheral gliomas according to histopathology and mutation characteristics (WHO, grade I) and infiltrating glioma (WHO, grade II-IV), which in turn includes glioblastoma (accounting for 60-70% of all malignant gliomas), anaplastic astrocytoma (accounting for 10-15%), anaplastic oligodendroglioma and anaplastic oligoastrocytoma (10%), and some other less com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/155A61P35/00A61B18/00
CPCA61B18/00A61K31/155A61P35/00
Inventor 孙敏轩杨帆周元帅杨姣黄煜伦李炎炎乔治·塞吉亚迪斯赵容川孟星君撒小涵欧阳楠
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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