Synergistic inhibition of Escherichia coli with phenol saffron red and ultrasound
A technology of phenol saffron and Escherichia coli, which is applied in the application field of the sound sensitizer phenol saffron combined with ultrasound to inhibit the activity of Escherichia coli, which can solve the problem of irrational use of antibiotics, enhanced bacterial resistance, and the number of patients Increase and other problems, to achieve the effect of overcoming bacterial resistance, high effectiveness, and promoting absorption
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Embodiment 1
[0021] Embodiment 1: the preparation of experimental solution
[0022] (1) Preparation of liquid medium
[0023] Weigh 0.2g of peptone, 0.2g of yeast extract powder, and 0.1g of sodium chloride, put them in a 50mL conical flask and add water to 20mL; seal the mouth of the bottle with a cotton plug, wrap it with newspaper, and tie it tightly with a cotton rope at 121°C under high pressure. Steam sterilized for 15 minutes before use.
[0024] (2) Preparation of solid medium
[0025] Weigh 6.67g nutrient agar respectively, put them into two 250mL conical flasks, add water to 200mL respectively, heat to dissolve, seal the bottle mouths with rubber stoppers, wrap them in newspapers and tie them tightly with cotton thread, and steam under high pressure at 121°C Sterilize for 15 minutes.
[0026] (3) Preparation of normal saline
[0027] Weigh 9g of sodium chloride, dissolve it in distilled water, and set the volume in a 1000mL volumetric flask to obtain 0.9% NaCl physiological s...
Embodiment 2
[0032] Example 2: Effect of compound concentration on its synergistic ultrasonic inhibition of Escherichia coli
[0033] Take eight 5mL capped centrifuge tubes, wrap them in newspaper and tie them tightly with cotton rope. Take 8 empty Erlenmeyer flasks, seal the mouths of the flasks with cotton plugs, wrap them in kraft paper and tie them tightly with cotton thread. Take 100mL of normal saline and put it in a 100mL Erlenmeyer flask. The above items were sterilized by high-pressure steam at 121°C for 15 minutes before use. After the ultra-clean bench was sterilized by ultraviolet for 30 minutes, the experiment was carried out around the lit alcohol lamp. Under sterile conditions, take 8 sterilized 5mL centrifuge tubes with caps. Add 1960 μL, 1950 μL, 1930 μL, 1910 μL of physiological saline, 0 μL, 10 μL, 30 μL, 50 μL and 1×10 7 cfu / mL bacterial suspension 40μL. The final concentration of the compound was 0mg / L, 5mg / L, 15mg / L, 25mg / L. After marking the centrifuge tubes, s...
Embodiment 3
[0036] Example 3: Effect of Ultrasonic Irradiation Time on Compound Synergistic Ultrasonic Inhibition of Escherichia coli
[0037] Under sterile conditions, take 8 sterilized 5mL centrifuge tubes with lids, and add 1×10 7 cfu / mL bacterial suspension 40 μL, compound solution 30 μL, each group was supplemented with normal saline to 2 mL. After marking the centrifuge tubes, seal each centrifuge tube with a parafilm to prevent water ingress and bacterial contamination during the ultrasonic process. Each group was irradiated with ultrasound. During the ultrasound process, the water temperature was controlled at 20°C, and the contact position between the centrifuge tube and the ultrasound probe was adjusted in time to ensure that the centrifuge tubes at the same ultrasound probe received uniform ultrasound irradiation. The water level is controlled at 70mm during ultrasonication.
[0038] The ultrasound group took samples of 100 μL at 20 min, 40 min, and 60 min, followed the steps...
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