Method for collecting mummified aphid of aphidius gifuensis in experimental investigation

A technology of Myzus persicae and Aphids, applied in animal husbandry and other directions, can solve the problems of death, easy breakage, affecting the accuracy of test results, etc., and achieves the effect of simple operation method and convenient research work.

Inactive Publication Date: 2020-08-18
TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the current research, there are deficiencies: when the aphids are parasitized by the wasps, with the growth and development of the larvae of the wasps, the internal organs of the aphids are completely eaten by the larvae of the wasps, and the aphids die; Bee larvae begin to spin silk and pupate, first bite a hole on the abdomen of the aphid, spin the silk to stick the empty shell of the aphid to the surface of the leaf or other objects and gradually form a dead aphid; the dead aphid stuck to the substrate is not easy to fall off, and the dead aphid The body is relatively soft, especially the junction between the abdomen of the aphid and the substrate is thin, and it is easy to be broken during the collection process. Once there is a breach in the abdomen, the development of the pupae in the aphid will be affected, resulting in a significant decline in eclosion rate, which will seriously Affect the accuracy of test results

Method used

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Embodiment Construction

[0016] The present invention is described in further detail below by embodiment, and embodiment is only used for illustrating the present invention, does not limit the scope of the present invention.

[0017] The method for collecting Aphids aphids in the experimental research of the present embodiment includes the following collection steps:

[0018] Step 1. Spread the wet filter paper in a disposable petri dish, and spread round leaves of the same size as the petri dish on the filter paper, keep the front of the leaf and the filter paper attached to prevent aphids from drilling into the front of the leaf, and the back of the leaf is facing upwards. Make sure that there is no clear water in the petri dish and on the leaves;

[0019] Step 2, insert the 2-3 instar parasitic aphids parasitized by Aphididae, cover the lid of the petri dish, and punch small holes on the lid of the petri dish to keep it ventilated;

[0020] Step 3, place the culture dish at room temperature or in ...

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Abstract

The invention relates to the field of insect collection, and particularly relates to a method for collecting a mummified aphid of aphidius gifuensis in the experimental investigation. According to themethod, wet filter paper is placed in a disposable culture dish, a round leave with the same size with the culture dish is paved on the wet filter paper, a parasitic aphid on the age of 2 to 3, whichis parasitized by aphidius, is placed on the round leave in the disposable culture dish, a culture dish cover is covered, and a small hole with a specification of 1mm is formed on the culture dish cover so as to keep ventilation. The method disclosed by the invention is simple in operation method and is convenient for the research work of a science researcher by placing the culture dish at the room temperature or in an artificial climate box.

Description

technical field [0001] The invention relates to the field of insect collection, in particular to a method for collecting aphids of Aphids persicae in experimental research. Background technique [0002] Scholars in our country began to conduct research on Aphids persicae since the 1970s. Although the large-scale breeding of A. There is still a lot of research space and necessity. Aphid wasps are a kind of parasitic natural enemy insects that specialize in parasitic aphids. There are many kinds of Aphids wasps, important species are Aphis persica, A. Aphids, Aphids, Aphids, Aphids, etc. Among them, Aphids aphidis is a very important dominant species. Aphididae is an important parasitic natural enemy. The wasp can parasitize a variety of aphids, mainly cabbage aphid, radish aphid, tobacco aphid, etc. It is one of the important natural enemies of aphids, has important utilization value, and plays an important role in the biological control of natural enemies. [0003] Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李成军邱睿李小杰李淑君白静科陈秀华王红军赵钧陈玉国金磊郭传滨刘畅
Owner TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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