Tissue culture and rapid propagation method of iris japonica 'Butterfly Dreams'

A technique for tissue culture rapid propagation and butterfly flower, applied in the field of botany, can solve the problem of inappropriate tissue culture of butterfly flower 'Dream Butterfly', and achieve the effect of remarkable technological progress and fast speed.

Active Publication Date: 2020-08-25
SHANGHAI BOTANICAL GARDEN
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  • Tissue culture and rapid propagation method of iris japonica 'Butterfly Dreams'
  • Tissue culture and rapid propagation method of iris japonica 'Butterfly Dreams'
  • Tissue culture and rapid propagation method of iris japonica 'Butterfly Dreams'

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Embodiment 1

[0028] like Figure 1-9 As shown, the present invention provides a method for tissue culture and rapid propagation of pansy 'Dream Butterfly', comprising the following steps: Step 1: material selection

[0029] From the first ten days of March to the middle ten days of March, select the young flower stems of 'Mengdie' that are healthy and free from diseases and insect pests, and cut off the whole flower stems for later use.

[0030] Step 2: Material Sterilization

[0031] Trim and clean the intercepted flower stems, soak and stir with detergent, rinse with running water for 2 hours, transfer to a super-clean workbench, soak in alcohol with a mass percentage concentration of 70% for 30 seconds, rinse with sterile water once, repeat three times, and then wash with mass percentage Concentration of 0.2% HgCl 2 Shake for 7 minutes, wash with sterile water three times for later use.

[0032] Step 3: Callus induction

[0033] The materials in step 2 were cut sideways and placed v...

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Abstract

The present invention provides a tissue culture and rapid propagation method of iris japonica 'Butterfly Dreams' and belongs to the field of botany. The tissue culture and rapid propagation method ofiris japonica 'Butterfly Dreams' comprises the following steps: (1) disinfection of explants; (2) induction of callus; (4) differentiation of the callus into cluster buds; (5) subsequent proliferationof the cluster buds; (6) seedling strengthening and rooting of the cluster buds; and (7) tissue culture seedling exercising and transplanting. Young and tender flower stems of the iris japonica are used as the explants, the callus is differentiated to form seedlings by indirect induction which is faster than a direct induction for propagation, a large number of new plants are obtained in a shortperiod of time to maintain excellent shapes of a parent plant and can be used as a transgenic receptor system, and the method lays a foundation for further popularization and application of the variety and transgenosis research.

Description

technical field [0001] The invention belongs to the field of botany and relates to a tissue culture technique, in particular to a tissue culture and rapid propagation method for pansy 'Mengdie'. Background technique [0002] Iris has a long history of cultivation, with rich flower colors and peculiar flower shapes, and has important ornamental value and ornamental value. China has long relied on the introduction of iris from Japan, the United States, the Netherlands and other places, but there are currently problems such as complicated introduction procedures and high prices, which have greatly affected the promotion and application of iris in China. [0003] The butterfly flower 'Mengdie' is a new variety of butterfly flower independently cultivated by the Shanghai Botanical Garden. Branch propagation can obtain new plants with flowering ability in a relatively short period of time, and can maintain the specificity of the species. It is still the most common method of iris...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 张蕾蕾肖月娥奉树成于凤阳王翠梅
Owner SHANGHAI BOTANICAL GARDEN
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