A method and application of poisonous Amanita species identification based on dna micro-barcoding technology
A micro-barcoding and species identification technology, applied in the field of food quality and safety testing, can solve problems such as damage and inability to extract genes
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[0022] The present invention will be further described by way of examples, but the present invention is not limited to the following examples.
[0023] This example is to evaluate the accuracy of DNA micro-barcoding amplification efficiency and identification of toxic Amanita by the following tests.
[0024] 1. Experimental materials
[0025] The names of 43 mushroom samples of 33 species of Amanita used in this study are shown in Table 1. Among them, Amanita pseudoglandum was provided by the Chinese Center for Disease Control and Prevention, and the other samples of Amanita were provided by Professor Chen Zuohong of Hunan Normal University for morphological identification. ITS , Large Subunit Ribosomal LSU ), the Second Largest Subunit of RNA Polymerase II (the Second Largest Subunit of RNA Polymerase II, RPB2 ), β-tubulin ( β-tubulin ) 4 DNA barcodes to identify its species authenticity.
[0026] Table 1 Information of collected Amanita samples
[0027] s...
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