A microbial detection system

A technology of microbial detection and micro-channel structure, which is applied in enzymology/microbiology devices, biomass post-treatment, biomass pre-treatment, etc., can solve the problem of large pressure difference of continuous phase fluid, difference in filling effect of micro-reaction chamber, and difficulty in distinguishing and other problems to achieve the effects of reducing risks, preventing droplet fusion defects, and improving distribution effects

Active Publication Date: 2021-07-16
湖南中瑞互信医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the resistance of the microchannel itself and the local barrier, there is a large pressure difference in the continuous phase fluid flowing between the upstream and downstream of the distribution channel, which will lead to a huge difference in the filling effect of the micro-reaction chamber at the upstream and downstream of the distribution channel.
[0005] In addition, in the process of fluorescence detection of the amplified micro-reaction chamber, the reflection and refraction of the incident excitation light often cause strong background interference; Due to the non-directional nature of the fluorescence emitted by the stimulated micro-droplets in the reaction chamber, some of the fluorescence signals between adjacent micro-wells often pass through the hole wall and are mixed with the fluorescent signals from adjacent droplets, making it difficult to distinguish The problem

Method used

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  • A microbial detection system
  • A microbial detection system
  • A microbial detection system

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Effect test

Embodiment 1

[0085] A method for preparing a vertical microbial detection chip is provided, the chip 10 only includes a double-layer structure of a cover layer 1 and a base layer 2, and the cover layer 1 is provided with an inlet through hole 11 and an outlet through hole 12; The substrate layer 2 is provided with a non-penetrating trough-like microchannel structure, the microchannel structure includes a sample inlet trough 21, a sample outlet trough 22, a delivery channel 23 bent and extended between the two, and connected to the delivery channel. The accommodating cavity 24 of the channel horizontal section and the side flow channel 25 connecting the bottom of the accommodating cavity 24 and the next horizontal section; the specific steps are as follows:

[0086] Step 1, select a cover sheet layer and a substrate layer with the same shape and size, wherein the material of the sheet layer can be conventional materials in the field, such as glass, PDMS, etc.;

[0087] Step 2, preparing a m...

Embodiment 2

[0102] Provide a kind of preparation method of vertical microbial detection chip, described chip 10 comprises cover sheet layer 1, base sheet layer 2 and and the structural layer 3 that is interposed between cover sheet layer 1 and base sheet layer 2, described cover The sheet layer 1 and the substrate layer 2 have an overlapping non-through microchannel structure; the cover sheet layer 1 has a through inlet through hole 11 and an outlet through hole 12; the base layer 2 has a corresponding non-through sample inlet Groove 21 and sample outlet groove 22; Described structural layer 3 comprises the part that overlaps with the microchannel structure on cover sheet layer 1 and substrate layer 2, and the sample feeding corresponding to described inlet through hole 11 and outlet through hole 12 The middle groove 31 and the middle groove 32 of the sample; only the microchannel structure of the structural layer 3 includes a middle layer side flow channel 35 for forming a lateral flow; t...

Embodiment 3

[0119] see Figure 11-19 , the chip 10 has eaves 242; specifically, it may be a double eaves 242 structure, or a single eaves 242 downstream of the accommodation chamber and a guide 243 upstream of the accommodation chamber, similar to the structure described above. Wherein, the eaves portion 242 and the guide portion 243 are both composed of the cover sheet layer 1 , the base sheet layer 2 and the structural layer 3 .

[0120] The sidewall of the middle layer delivery channel 33 has stronger hydrophilicity than the sidewalls of the water delivery channel on the cover sheet layer 1 and the substrate layer 2, so as to allow the middle layer side flow channel 35 to have a relatively small In the case of a cross-sectional area of ​​35, the same lateral flow intensity is provided, so that the induction effect on micro-droplets will not be reduced due to the reduction of the cross-sectional area of ​​the side flow channel 35 in the middle layer.

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Abstract

The invention relates to a preparation method of a vertical microbiological detection chip, through which a differentiated mask is used to form a penetrating and / or non-penetrating microchannel structure on each sheet; then the materials of each sheet are combined to obtain The water transport channel and the accommodation chamber for containing micro-droplets on a single sheet, or the combined transport channel and combined accommodation chamber formed by a combination of multiple sheets, the chip prepared by the present invention also has the ability to induce micro-fluid The droplet enters the side flow channel of the holding chamber, which can have the same depth as the holding chamber, or is only provided in the middle layer, thereby having a depth smaller than that of the holding chamber, by setting the microchannel structure of the middle layer at Compared with the microchannel structure of other sheets, it has stronger hydrophilic characteristics, allowing the use of a reduced cross-sectional area of ​​the lateral flow channel to achieve the same lateral flow intensity.

Description

technical field [0001] The invention relates to a biological detection device, in particular to a microbial detection system for performing specific DNA detection of microorganisms such as viruses and bacteria through PCR reactions. Background technique [0002] The digital PCR chip based on micro-droplets is one of the most advanced nucleic acid quantitative detection methods. Compared with traditional PCR technology, digital PCR dilutes the solution containing target genes, primers, polymerases, etc., and divides them into dozens to dozens Tens of thousands of tiny, independent reactors, so that the number of nucleic acid templates in each reactor is less than or equal to 1, and each reactor is subjected to traditional PCR amplification and fluorescence detection. The reactor containing the target gene is marked as 1, and the reactor without the target gene is marked as 0. According to the relative ratio and the volume of the reactor, the nucleic acid concentration of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00
CPCC12M23/16C12M23/20C12M41/36
Inventor 郑同玉
Owner 湖南中瑞互信医疗科技有限公司
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