Vaccine vector prepared based on anionic polymer and derivatives thereof

An anionic polymer and vaccine carrier technology, applied in the field of vaccine carrier and its preparation based on anionic polymer and its derivative materials, can solve the problem that it is difficult to make accurate evaluation of adjuvant effect, can not induce Th1 type immune response, Local adverse reactions hypersensitivity and other issues, to achieve the effect of simple and rapid preparation method, low toxicity and mild conditions

Active Publication Date: 2020-09-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it cannot stimulate intracellular immune response, cannot induce Th1-type immune response, and there is a risk of potential local adverse reactions and hypersensitivity reactions, and the quality is difficult to control and it is difficult to make an accurate evaluation of the effect of adjuvants
Although the inability of aluminum adjuvants to effectively induce Th1 responses limits its scope of application, its safety and effectiveness in humans has been proven by time, so we believe that it will be very useful to construct a vaccine carrier with aluminum hydroxide as the core. potential

Method used

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  • Vaccine vector prepared based on anionic polymer and derivatives thereof
  • Vaccine vector prepared based on anionic polymer and derivatives thereof
  • Vaccine vector prepared based on anionic polymer and derivatives thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0072] Preparation of aluminum hydroxide nanoparticles based on γ-polyglutamic acid material: add 125μl 1mg / ml γ-polyglutamic acid to 345μl 80mmol / L Hepes buffer with pH 8 and mix well, and draw 550μl 1.76 mmol / L The aluminum sulfate solution of L is added to the above-mentioned solution, sonicated for 5 minutes, and the power is 120w, and it is obtained.

Embodiment 2

[0074] Preparation of aluminum hydroxide nanoparticles based on γ-polyglutamic acid material: add 120μl of 8mg / ml γ-polyglutamic acid to 360μl of 100mmol / L Hepes buffer with a pH of 8, mix well, and draw 555μl of 17.56 mmol / L The aluminum sulfate solution of L is added to the above solution, ultrasonicated for 10 minutes, and the power is 150w, and it is obtained.

Embodiment 3

[0076] Preparation of aluminum hydroxide nanoparticles-OVA based on γ-polyglutamic acid material: Add 130μl of 20mg / ml γ-polyglutamic acid to 370μl of 100mmol / L Hepes buffer with a pH of 8 and mix well, and 450μl of pipette 2.16 Mix the mmol / L aluminum sulfate solution and 100μl 4mg / ml OVA solution uniformly, add them to the above solution, sonicate for 8min, power 100w, and get it.

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Abstract

The invention provides a vaccine vector prepared based on an anionic polymer and derivatives thereof, and provides a preparation method of the vaccine vector. Nanoparticles are compounded with aluminum salt through a series of anionic polymers and derivatives thereof to form aluminum hydroxide; and different antigen components are added in the preparation process to encapsulate the antigen. The prepared vaccine can be efficiently taken by antigen presenting cells, transferred to lymph nodes and induce antigen-specific immune response, and has a wide vaccine application prospect.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to a vaccine carrier based on anionic polymer and its derivative materials and a preparation method thereof. Background technique [0002] Vaccination is one of the great victories of modern medicine. It is also an effective means of preventing and eradicating most diseases. It is used to control many infectious diseases and even eradicate smallpox. General vaccines are mainly attenuated or inactivated vaccines with high immunogenicity but low safety. The current research is mainly focused on subunit vaccines because they are more pure and safe. However, compared with attenuated vaccines, the increase in safety is accompanied by a decrease in immunogenicity. Therefore, adjuvants have become a vital component in vaccines to enhance the lasting and effective immune effect of subunit vaccines. The use of vaccine delivery vehicles can induce an effective immune response and provide impro...

Claims

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Application Information

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IPC IPC(8): A61K47/02A61K47/34A61K47/36A61K39/39
CPCA61K9/143A61K9/146A61K39/39
Inventor 孙逊张志荣龚涛侯盈盈白淑婷
Owner SICHUAN UNIV
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