A variable region sequence of a specific anti-pyraclostrobin antibody and its recombinant full-length antibody

Abstract

The invention discloses a variable region sequence of a specific anti-pyraclostrobin antibody, and the amino acid sequence of the gene encoding the variable region of the heavy chain is shown in SEQ ID NO:2. The invention also discloses a recombinant full-length antibody against pyraclostrobin, including heavy chain constant region, heavy chain variable region, light chain constant region and light chain variable region, and the amino acid of the gene encoded by the heavy chain variable region The sequence is shown as SEQ ID NO:2. The invention also discloses an antibody expression plasmid. The sequence gene obtained in the present invention is connected to the expression vector containing the heavy chain constant region gene and the light chain constant region gene respectively, and the recombinant full-length antibody is obtained by expressing the mammalian cell of the double plasmid system, and the recognition of the mouse parent antibody The activity is consistent, and the anti-pyraclostrobin antibody can be stably produced in large quantities, providing a reliable core reagent for various immunoassay methods for the detection of pyraclostrobin residues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation and application of a variable region sequence of a specific anti-pyraclostrobin antibody and its recombinant full-length antibody. Background technique [0002] Pyraclostrobin is a new type of strobilurin fungicide developed by BASF (BASF) in 1993. It prevents electron transfer between cytochrome B and C1, inhibits mitochondrial respiration and inhibits The production of ATP eventually leads to the death of pathogenic fungi that cannot carry out normal physiological metabolism. As an environmentally friendly fungicide, pyraclostrobin is less harmful to non-target organisms, but it has been reported to have certain mitochondrial toxicity and neurotoxicity to silkworms and zebrafish. Therefore, pyraclostrobin should be controlled in agricultural production. The use of bacteriocin. According to the latest national standard (GB 2763-2019), th...

AI Technical Summary

Problems solved by technology

[0005] However, hybridoma cells have a complex genetic background and gene deletions / mutations that occur during long-term passage and thawing and freezing, which can easily lead to differences in the ability of monoclonal antibodies obtained from different batches to specifically recognize antigens, affecting rapid immunoassay methods stability

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