A variable region sequence of a specific anti-thiacloprid antibody and its recombinant complete antibody

A complete antibody and variable region technology, applied in the field of genetic engineering, can solve problems such as differences between antibody batches, inability of cell lines to produce effective antibodies, and easy loss of effective genes, etc., to achieve high selectivity, high sensitivity, and guaranteed recognition activity Effect

CN112111010BActive Publication Date: 2022-04-19ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-04-19

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Abstract

The invention discloses a variable region sequence of a specific anti-thiacloprid antibody. The amino acid sequence of the gene encoding the heavy chain variable region is shown in SEQ ID NO:2. The present invention also discloses an anti-thiacloprid recombinant complete antibody, including heavy chain constant region, heavy chain variable region, light chain constant region and light chain variable region, the amino acid sequence of the gene encoding the heavy chain variable region is as follows: Shown in SEQ ID NO:2. The invention also discloses an antibody expression plasmid. The sequence genes obtained in the present invention are respectively connected to the expression vectors containing the heavy chain constant region gene and the light chain constant region gene, and the recombinant complete antibody is obtained by using the mammalian cell expression of the double plasmid system, which ensures the recognition of the mouse parent antibody activity, and enables the stable production of anti-thiacloprid antibodies in large quantities, and provides reliable core reagents for various immunoassay methods for the detection of thiacloprid residues.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation of a variable region sequence of a specific anti-thiacloprid antibody and its recombinant complete antibody. Background technique

[0002] Thiacloprid (3-(6-chloro-3-methylpyridine)-1,3-thiazolidine-2-cyanamide) is a new type of chloronicotinoid insecticide with a thiazole ring. It has a unique structure and better performance than traditional neonicotinoid insecticides, and is widely used in the prevention and control of pests with piercing-sucking mouthparts and chewing mouthparts on crops. Thiacloprid has strong systemic and osmotic effects, so it will be distributed in various parts of crops, and because of its strong water solubility and long half-life, it can easily enter surface water and soil through rainfall and irrigation. Studies have shown that there are high levels of thiacloprid residues in surface water, sediment and soil, a...

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Embodiment Construction

[0021] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative work should belong to the protection scope of the present invention. If there is no special description, the experimental methods in the embodiments are all for the conventional method.

[0022] The anti-thiacloprid recombinant complete antibody involved in the present invention includes a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the...