A variable region sequence of a specific anti-thiacloprid antibody and its recombinant complete antibody

Abstract

The invention discloses a variable region sequence of a specific anti-thiacloprid antibody. The amino acid sequence of the gene encoding the heavy chain variable region is shown in SEQ ID NO:2. The present invention also discloses an anti-thiacloprid recombinant complete antibody, including heavy chain constant region, heavy chain variable region, light chain constant region and light chain variable region, the amino acid sequence of the gene encoding the heavy chain variable region is as follows: Shown in SEQ ID NO:2. The invention also discloses an antibody expression plasmid. The sequence genes obtained in the present invention are respectively connected to the expression vectors containing the heavy chain constant region gene and the light chain constant region gene, and the recombinant complete antibody is obtained by using the mammalian cell expression of the double plasmid system, which ensures the recognition of the mouse parent antibody activity, and enables the stable production of anti-thiacloprid antibodies in large quantities, and provides reliable core reagents for various immunoassay methods for the detection of thiacloprid residues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation of a variable region sequence of a specific anti-thiacloprid antibody and its recombinant complete antibody. Background technique [0002] Thiacloprid (3-(6-chloro-3-methylpyridine)-1,3-thiazolidine-2-cyanamide) is a new type of chloronicotinoid insecticide with a thiazole ring. It has a unique structure and better performance than traditional neonicotinoid insecticides, and is widely used in the prevention and control of pests with piercing-sucking mouthparts and chewing mouthparts on crops. Thiacloprid has strong systemic and osmotic effects, so it will be distributed in various parts of crops, and because of its strong water solubility and long half-life, it can easily enter surface water and soil through rainfall and irrigation. Studies have shown that there are high levels of thiacloprid residues in surface water, sediment and soil, a...

AI Technical Summary

Problems solved by technology

Among the many recombinant antibody expression systems, the mammalian cell expression system can guide the correct folding of recombinant antibodies and provide accurate various post-translational processing and modification functions, so it can stably produce recombinant antibodies with the same activity as the parental antibody, which not only confirms The accuracy of the variable region sequence of the parental antibody can also solve many problems faced by conventional antibody production, such as the batch-to-batch difference of traditional antibodies, and the effective genes are easily lost during the passage and preservation of cell lines, resulting in the inability of cell lines to produce effective antibodies

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