A variable region sequence of a specific anti-thiamethoxam antibody and its recombinant full-length antibody

Abstract

The invention discloses a variable region sequence of a specific anti-thiamethoxam antibody. The amino acid sequence of the heavy chain variable region coding gene is shown in SEQ ID NO:2. The invention also discloses an anti-thiamethoxam recombinant full-length antibody, which includes a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the gene encoding the heavy chain variable region is: As shown in SEQ ID NO:2. The invention also discloses an antibody expression plasmid. The sequence genes obtained in the present invention are connected to the expression vectors containing the heavy chain constant region gene and the light chain constant region gene respectively, and the recombinant full-length antibody is obtained by expressing the mammalian cells of the dual-plasmid system, which ensures the stability of the mouse parent antibody. The recognition activity enables the stable production of anti-thiamethoxam antibodies in large quantities, and provides reliable core reagents for various immunoassay methods for the detection of thiamethoxam residues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation of a variable region sequence of a specific anti-thiamethoxam antibody and a recombinant full-length antibody thereof. Background technique [0002] Neonicotinoid insecticides have become the fastest growing insecticides in the global market and are widely used in more than 120 countries around the world. This type of insecticide inhibits the acetylcholinesterase receptor of the central nervous system of the pest, blocks the normal conduction of the central nervous system of the pest, and causes the paralysis of the pest to death. etc. have a good control effect. Thiamethoxam (3-(2-chloro-1,3-thiazol-5-ylmethyl)-5-methyl-1,3,5-oxadiazin-4-ylidene(nitro)amine) is A second-generation neonicotinoid insecticide with a new structure has strong systemic and osmotic effects, so it will be distributed in various parts of the crop, and because of ...

AI Technical Summary

Problems solved by technology

Among the many recombinant antibody expression systems, the mammalian cell expression system can guide the correct folding of recombinant antibodies and provide accurate various post-translational processing and modification functions, so it can stably produce recombinant antibodies with the same activity as the parental antibody, which not only confirms The accuracy of the variable region sequence of the parental antibody can also solve many problems faced by conventional antibody production, such as the batch-to-batch difference of traditional antibodies, and the effective genes are easily lost during the passage and preservation of cell lines, resulting in the inability of cell lines to produce effective antibodies

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